Messenger RNA from Staphylococcus aureus that specifies macrolide-lincosamide-streptogramin resistance. Demonstration of its conformations and of the leader peptide it encodes.
Messenger RNA from Staphylococcus aureus that specifies macrolide-lincosamide-streptogramin resistance. Demonstration of its conformations and of the leader peptide it encodes.
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来自金黄色葡萄球菌的信使 RNA，指定大环内酯-林可酰胺-链霉素耐药性。
DOI：
10.1016/0022-2836(85)90061-0
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发表时间：
1985
影响因子：
5.6
通讯作者：
Weisblum,B
中科院分区：
文献类型：
--
作者：
Mayford,M;Weisblum,B
通过对体内合成的mRNA进行核酸酶S1作图和对体外合成的（5′-γ-32 P）-末端标记转录本进行核酸酶T1作图，确定了质粒pE 194编码的23 SrRNA腺嘌呤甲基化酶的+1转录起始位点。用S1和眼镜蛇毒核酸酶作为mRNA构象的探针，通过部分消化thein vitrotranscripts，分析延伸到揭示单链和双链区域，分别对应于假定的新生mRNA的活性和非活性构象的关键茎和环。根据诱导模型，从非活性构象到活性构象的转变涉及mRNA二级结构的破坏，这反过来又是基于与红霉素复合的核糖体对关键茎序列之一的长期占用。关键茎序列的核糖体占据是由于存在编码19个氨基酸残基的“前导”肽的一部分的开放阅读框。从甲基化酶结构基因上游控制区的核苷酸序列推断的该肽的存在，在体内作为与大肠杆菌β-半乳糖苷酶的翻译融合的一部分被证明，其中通过微测序方法直接分析的融合蛋白的N-末端序列的前四个氨基酸残基被发现包含N-末端氨基酸2至5，Gly-Ile-Phe-Ser，预测的前导肽。
The + 1 site for transcription initiation of the inducible 23 S rRNA adenine methylase encoded by plasmid pE194 was determined experimentally by nuclease S1mapping of mRNA synthesizedin vivo, and by nuclease T1mapping of (5′-γ-32P)-end-labeled transcripts synthesizedin vitro. By partial digestion of thein vitrotranscripts using S1and cobra venom nuclease as probes of mRNA conformation, the analysis was extended to reveal single-stranded and double-stranded regions, respectively, which correspond to the critical stems and loops postulated for active and inactive conformations of the nascent mRNA. According to the model for induction, the transition from inactive to active conformation involves disruption of mRNA secondary structure which, in turn, is predicated on protracted occupancy by ribosomes complexed with erythromycin of one of the critical stem sequences. Ribosome occupancy of the critical stem sequence is due to the presence of an open reading frame that encodes part of a 19 amino acid residue “leader” peptide. The existence of this peptide, deduced from the nucleotide sequence of the control region upstream from the methylase structural gene, was demonstratedin vivoas part of a translational fusion withEscherichia coliβ-galactosidase in which the first four amino acid residues of the N-terminal sequence of the fusion protein, analyzed directly by the microsequencing method, were found to comprise N-terminal amino acids 2 through 5, Gly-Ile-Phe-Ser, predicted for the leader peptide.
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使用气相测序仪进行高灵敏度测序。
影响因子：
--
作者：
M. Hunkapiller;R. Hewick;W. Dreyer;L. Hood
通讯作者：
L. Hood
影响因子：
14.9
作者：
LOCKARD, RE;KUMAR, A
通讯作者：
KUMAR, A
抗红霉素金黄色葡萄球菌 23S 核糖体核糖核酸中可诱导甲基化核苷酸序列的结构。
影响因子：
2.9
作者：
C. Lai;J. Dahlberg;B. Weisblum
通讯作者：
B. Weisblum
大肠杆菌 K12 β-半乳糖苷酶的纯化、组成和分子量。
影响因子：
4.8
作者：
G. Craven;E. Steers;C. Anfinsen
通讯作者：
C. Anfinsen
金黄色葡萄球菌中红霉素诱导的耐药性：涉及抗生素类别的调查
影响因子：
3.2
作者：
B. Weisblum;V. Demohn
通讯作者：
V. Demohn