A Salt-Signaling Network Involving Ethylene, Extracellular ATP, Hydrogen Peroxide, and Calcium Mediates K(+)/Na(+) Homeostasis in Arabidopsis.
A Salt-Signaling Network Involving Ethylene, Extracellular ATP, Hydrogen Peroxide, and Calcium Mediates K(+)/Na(+) Homeostasis in Arabidopsis.
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拟南芥中涉及乙烯、细胞外 ATP、过氧化氢和钙的盐信号网络介导 K/Na 稳态
DOI：
10.3390/ijms21228683
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发表时间：
2020-11-17
影响因子：
5.6
通讯作者：
Deng S
中科院分区：
文献类型：
--
作者：
Lang T;Deng C;Yao J;Zhang H;Wang Y;Deng S
这项工作旨在研究盐信号分子的相互作用，即，乙烯、细胞外ATP（eATP）、H2 O2和胞内Ca 2+（[Ca 2 +]cyt）对拟南芥K+/Na+稳态的调节。在无盐条件下，eATP的存在缩短了Col-0下胚轴的长度。此外，eATP显著降低了盐胁迫下Col-0植株的相对电解质渗漏率，延长了根长，但对乙烯不敏感突变体etr 1 -1和ein 3 - 1 eil 1 -1影响不明显。稳态离子通量动力学表明，外源1-氨基环丙烷-1-羧酸（ACC，乙烯前体）和eATP-Na 2（eATP供体）显着增加Na+的挤出和抑制K+的损失在短期NaCl处理。ACC能显著增强盐诱导的H2 O2和胞浆Ca ~（2+）的荧光强度。我们的qPCR数据显示，在NaCl胁迫12 h期间，ACC的应用增加了AtSOS 1和AtAHA 1的表达，它们分别编码质膜（PM）Na+/H+反向转运蛋白（SOS 1）和H+-ATP酶（H+泵）。此外，eATP显著增加AtEIN 3、AtEIL 1和AtETR 1的转录，ACC处理Col-0根后，AtRbohF和AtSOS 2/3表达上调，这两个基因分别直接参与H2 O2和Ca 2+信号转导途径。简单地说，乙烯是由eATP触发的，eATP是一种新的上游信号成分，然后激活并加强H2 O2和Ca 2+信号通路，以维持盐度下K+/Na+的稳态。
This work aimed at investigating the interactive effects of salt-signaling molecules, i.e., ethylene, extracellular ATP (eATP), H2O2, and cytosolic Ca2+ ([Ca2+]cyt), on the regulation of K+/Na+ homeostasis in Arabidopsis thaliana. The presence of eATP shortened Col-0 hypocotyl length under no-salt conditions. Moreover, eATP decreased relative electrolyte leakage and lengthened root length significantly in salt-treated Col-0 plants but had no obvious effects on the ethylene-insensitive mutants etr1-1 and ein3-1eil1-1. Steady-state ionic flux kinetics showed that exogenous 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) and eATP-Na2 (an eATP donor) significantly increased Na+ extrusion and suppressed K+ loss during short-term NaCl treatment. Moreover, ACC remarkably raised the fluorescence intensity of salt-elicited H2O2 and cytosolic Ca2+. Our qPCR data revealed that during 12 h of NaCl stress, application of ACC increased the expression of AtSOS1 and AtAHA1, which encode the plasma membrane (PM) Na+/H+ antiporters (SOS1) and H+-ATPase (H+ pumps), respectively. In addition, eATP markedly increased the transcription of AtEIN3, AtEIL1, and AtETR1, and ACC treatment of Col-0 roots under NaCl stress conditions caused upregulation of AtRbohF and AtSOS2/3, which directly contribute to the H2O2 and Ca2+ signaling pathways, respectively. Briefly, ethylene was triggered by eATP, a novel upstream signaling component, which then activated and strengthened the H2O2 and Ca2+ signaling pathways to maintain K+/Na+ homeostasis under salinity.
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