Temporary prenatal hyperglycemia leads to postnatal neuronal ‘glucose-resistance’ in the chicken hypothalamus
Temporary prenatal hyperglycemia leads to postnatal neuronal ‘glucose-resistance’ in the chicken hypothalamus
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暂时性产前高血糖导致鸡下丘脑产后神经元葡萄糖抵抗
DOI：
10.1016/j.brainres.2015.05.037
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发表时间：
2015
期刊：
影响因子：
2.9
通讯作者：
Plagemann A
中科院分区：
文献类型：
--
作者：
Tzschentke B;Bogatyrev S;Schellong K;Rancourt RC;Plagemann A
产前暴露可能对长期健康产生明显影响。在怀孕期间暴露于母体“糖尿病”会增加后代患“糖尿病”的风险。中枢神经对体重、食物摄入和新陈代谢的调节发生了错误。关键机制和具体干扰因素仍不清楚。由于鸡胚的独立发育，可以为明确地建立病因提供有价值的模型。本研究旨在探讨短暂性产前高血糖对鸡出生后下丘脑神经元葡萄糖敏感性的影响。为诱导鸡胚高血糖，从孵育第14 ~ 17天开始，每天用0.5 ml葡萄糖溶液（浓度为30 mmol/l）通过导管注入绒毛膜尿囊膜血管。出生后第21天，测定大鼠下丘脑脑片体重、体脂含量、血糖、神经电生理葡萄糖敏感性及葡萄糖转运蛋白表达。形态学和代谢参数未见明显变化。然而，神经元葡萄糖敏感性和葡萄糖转运蛋白表达强烈下降，表明产前获得性下丘脑“葡萄糖抵抗”。总之，暂时性的产前晚期高血糖会引起中枢葡萄糖感知的持久变化。产前葡萄糖处理鸡为研究“糖尿病”和相关疾病的早期中枢神经起源提供了一个有价值的新模型。
Prenatal exposures may have a distinct impact for long-term health. Exposure to maternal ‘diabesity’ during pregnancy increases offspring ‘diabesity’ risk,e.g.by malprogramming the central nervous regulation of body weight, food intake and metabolism. Critical mechanisms and concrete disrupting factors still remain unclear. Due to the independent development, from the mother, the chicken embryo could provide a valuable model to distinctively establish causal factors.Aim of this study was to determine effects of temporary prenatal hyperglycemia on postnatal hypothalamic neuronal glucose sensitivity in the chicken.To induce hyperglycemia in chicken embryos, 0.5 ml glucose solution (concentration 30 mmol/l) were daily administeredviacatheter into a vessel of the chorioallantoic egg membrane from days 14 to 17 of incubation. On day 21 of postnatal age, body weight, body fat content, blood glucose, neuroelectrophysiological glucose sensitivity as well as glucose transporter expression were determined in hypothalamic brain slices. No significant changes in morphometric and metabolic parameters were observed. However, strongly decreased neuronal glucose sensitivity and glucose transporter expression occurred, indicating prenatally acquired hypothalamic ‘glucose-resistance’. In conclusion, temporary late prenatal hyperglycemia induces lasting changes in central glucose sensing. The prenatally glucose-treated chicken provides a valuable new model for investigating early central nervous origins of ‘diabesity’ and related disorders.
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影响因子：
4.3
作者：
Verberne AJ;Sabetghadam A;Korim WS
通讯作者：
Korim WS
DOI：
10.1001/jama.2014.732
发表时间：
2014-02-26
期刊：
JAMA
影响因子：
--
作者：
Ogden CL;Carroll MD;Kit BK;Flegal KM
通讯作者：
Flegal KM
影响因子：
7.7
作者：
Levin, BE;Routh, VH;Dunn-Meynell, AA
通讯作者：
Dunn-Meynell, AA
影响因子：
2.4
作者：
Plagemann, Andreas;Roepke, Katharina;Dudenhausen, Joachim W.
通讯作者：
Dudenhausen, Joachim W.
影响因子：
7.7
作者：
Dabelea, D;Hanson, RL;Knowler, WC
通讯作者：
Knowler, WC