Characterization of a regulatory region in the N-terminus of rabbit kv1.3.
Characterization of a regulatory region in the N-terminus of rabbit kv1.3.
复制标题
兔 kv1.3 N 末端调节区的表征。
DOI：
10.1006/bbrc.1998.9122
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发表时间：
1998
影响因子：
3.1
通讯作者：
G. Desir
中科院分区：
文献类型：
--
作者：
X. Yao;Y. Huang;H. Kwan;P. Chan;A. S. Segal;G. Desir
兔Kv1.3的N-末端含有调节质膜中Kv1.3蛋白表达的信号序列。去除N-末端区域（aa 3 -39）产生表达的K+电流增加。在N-末端的进行性缺失表明，K电流升高所需的最短缺失是D 6 -34。由于功能性信号序列必须包括肽片段aa 6 -34的两端，其中带电残基密集分布，因此可以想象该N-末端信号序列与电荷或其相关的亲水性有关。通过氨基酸取代去除两个带电残基（31 R、33 E），将缺失构建体D3-27转化为D3-27*，有效地提高了表达电流的幅度，进一步表明带电残基的重要性。使用一个突变的发动蛋白和可溶性的N-末端肽，我们还发现，N-末端信号序列可能不通过内吞途径发挥作用。
The N-terminus of rabbit Kv1.3 contains a signal sequence which regulates expression of Kv1.3 proteins in the plasma membrane. Removal of an N-terminal region (aa3-39) produced an increase in expressed K+ current. Progressive deletion at the N-terminus demonstrated that the shortest deletion required for the elevation of K current is D6-34. Since the functional signal sequence must include both ends of the peptide segment aa6-34 where charged residues are densely distributed, it is conceivable that this N-terminal signal sequence is related to charge or its associated hydrophilicity. Removal of two charged residues (31R, 33E) through amino acid substitution which converts deletion construct D3-27 to D3-27* effectively raises the amplitude of expressed current, further indicating the importance of charged residues. With the use of a mutated dynamin and a soluble N-terminal peptide, we also revealed that the N-terminal signal sequence may not act through the endocytotic pathway.
兔肾中表达的格列本脲敏感、电压门控钾通道的分子克隆。
DOI：
10.1172/jci118700
发表时间：
1996
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Yao,X;Chang,AY;Boulpaep,EL;Segal,AS;Desir,GV
通讯作者：
Desir,GV