Rapid Methods for Antimicrobial Resistance Diagnostics.
Rapid Methods for Antimicrobial Resistance Diagnostics.
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DOI：
10.3390/antibiotics10020209
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发表时间：
2021-02-20
期刊：
影响因子：
--
通讯作者：
Prieto M
中科院分区：
文献类型：
--
作者：
Kaprou GD;Bergšpica I;Alexa EA;Alvarez-Ordóñez A;Prieto M
抗菌素耐药性(AMR)是公共卫生中最具挑战性的威胁之一；因此，对能够进行快速抗菌素敏感性检测(AST)的方法和技术的需求越来越大。临床微生物学中使用的解决AMR诊断和AST的传统方法和技术繁琐，周转时间(TAT)高，而且通常很昂贵。结果，开出了经验性的抗菌疗法，导致了AMR的传播，这反过来又导致了更高的死亡率和更高的医疗成本。这篇综述描述了当前尖端方法和技术的发展，按关键使能研究领域组织，通过利用AMR诊断工具的最新进展来对抗迫在眉睫的AMR威胁。首先，我们总结了解决AMR检测、监视和AST的常规方法。然后，我们回顾了最新的非常规方法及其在每个领域的进展，包括全基因组测序(WGS)、基质辅助激光解吸/电离飞行时间(MALDI-TOF)光谱、傅里叶变换红外(FTIR)光谱和微流控技术。下面，我们提供可用于AST的商业诊断平台的示例。最后，讨论了实施新兴概念以开发AMR诊断的改变范式的技术和方法的前景。
Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus, there is a growing demand for methods and technologies that enable rapid antimicrobial susceptibility testing (AST). The conventional methods and technologies addressing AMR diagnostics and AST employed in clinical microbiology are tedious, with high turnaround times (TAT), and are usually expensive. As a result, empirical antimicrobial therapies are prescribed leading to AMR spread, which in turn causes higher mortality rates and increased healthcare costs. This review describes the developments in current cutting-edge methods and technologies, organized by key enabling research domains, towards fighting the looming AMR menace by employing recent advances in AMR diagnostic tools. First, we summarize the conventional methods addressing AMR detection, surveillance, and AST. Thereafter, we examine more recent non-conventional methods and the advancements in each field, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and microfluidics technology. Following, we provide examples of commercially available diagnostic platforms for AST. Finally, perspectives on the implementation of emerging concepts towards developing paradigm-changing technologies and methodologies for AMR diagnostics are discussed.
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影响因子：
5.8
作者：
Axelsson, Carolina;Rehnstam-Holm, Ann-Sofi;Nilson, Bo
通讯作者：
Nilson, Bo
影响因子：
2.2
作者：
Amoako, Kingsley K.;Thomas, Matthew C.;Goji, Noriko
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
9.2
作者：
Brown BL;Watson M;Minot SS;Rivera MC;Franklin RB
通讯作者：
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影响因子：
5.2
作者：
Andrews, JM
通讯作者：
Andrews, JM