Nanofiber capsules for minimally invasive sampling of biological specimens from gastrointestinal tract.
Nanofiber capsules for minimally invasive sampling of biological specimens from gastrointestinal tract.
复制标题
用于胃肠道生物标本微创取样的纳米纤维胶囊。
DOI：
10.1016/j.actbio.2022.04.045
复制
发表时间：
2022-07-01
影响因子：
9.7
通讯作者：
Xie, Jingwei
中科院分区：
文献类型：
--
作者：
V. John, Johnson;McCarthy, Alec;Su, Yajuan;Ackerman, Daniel N.;Shahriar, S. M. Shatil;Carlson, Mark A.;Reid, St. Patrick;Santarpia, Joshua L.;Zhu, Wuqiang;Xie, Jingwei
准确和快速的护理点组织和微生物组采样对于癌症和传染病的早期检测至关重要，并且通常导致有效的早期干预和预防疾病传播。特别是，在西方世界，巴雷特和胃癌前病变的低患病率使得基于人群的内镜筛查不可行且成本低。在这里，我们报告了一种方法，可能是有用的预筛选一般人群在微创的方式使用可吞咽的，可再膨胀的，超吸收的，可回收的长方体和球体生产的静电纺丝，气体发泡，涂层和交联。长方体和球形物体的吸水能力显示为它们干质量的~6000%和~2000%。相比之下，未扩张的半圆形和方形羊膜显示其干质量的500%。此外，与未膨胀的支架相比，可吞咽的球体和长方体能够从溶液中收集和释放更多的细菌、病毒和细胞/组织。除此之外，与未膨胀的食管膜相比，膨胀的球体显示出更高的从食管内壁收集细胞的能力。总之，本研究中开发的胶囊可以提供一种从十二指肠、胃、食管和口咽部位收集生物样本的微创方法，可能导致许多疾病的及时准确诊断。
Accurate and rapid point-of-care tissue and microbiome sampling is critical for early detection of cancers and infectious diseases and often result in effective early intervention and prevention of disease spread. In particular, the low prevalence of Barrett’s and gastric premalignancy in the Western world makes population-based endoscopic screening unfeasible and cost-ineffective. Herein, we report a method that may be useful for prescreening the general population in a minimally invasive way using a swallowable, re-expandable, ultra-absorbable, and retrievable nanofiber cuboid and sphere produced by electrospinning, gas-foaming, coating, and crosslinking. The water absorption capacity of the cuboid- and sphere-shaped nanofiber objects is shown ~6000% and ~2000% of their dry mass. In contrast, unexpanded semicircular and square nanofiber membranes showed <500% of their dry mass. Moreover, the swallowable sphere and cuboid were able to collect and release more bacteria, viruses, and cells/tissues from solutions as compared with unexpanded scaffolds. In addition to that, an expanded sphere shows higher cell collection capacity from the esophagus inner wall as compared with the unexpanded nanofiber membrane. Taken together, the nanofiber capsules developed in this study could provide a minimally invasive method of collecting biological samples from the duodenal, gastric, esophagus, and oropharyngeal sites, potentially leading to timely and accurate diagnosis of many diseases.
登录
查看更多内容
影响因子：
3.9
作者：
Gastli N;Allain M;Lamarque D;Abitbol V;Billoët A;Collobert G;Coriat R;Terris B;Kalach N;Raymond J
通讯作者：
Raymond J
影响因子：
8
作者：
Lobato, MN;Loeffler, AM;Hopewell, PC
通讯作者：
Hopewell, PC
影响因子：
158.5
作者：
Spechler, SJ
通讯作者：
Spechler, SJ
DOI：
10.1002/adma.202003754
发表时间：
2020-10
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Chen S;McCarthy A;John JV;Su Y;Xie J
通讯作者：
Xie J
影响因子：
3.4
作者：
Guiney, William J.;Beaumont, Claire;Richards, Duncan
通讯作者：
Richards, Duncan