Converting 2D Nanofiber Membranes to 3D Hierarchical Assemblies with Structural and Compositional Gradients Regulates Cell Behavior.
Converting 2D Nanofiber Membranes to 3D Hierarchical Assemblies with Structural and Compositional Gradients Regulates Cell Behavior.
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DOI：
10.1002/adma.202003754
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Chen S;McCarthy A;John JV;Su Y;Xie J
描述了用于将二维（2D）电纺生物膜转化为具有结构和组成梯度的三维（3D）分级组件的新方法。孔径梯度是通过在气体发泡过程中掺入不同量的表面活性剂来调节不同层中的2D膜的膨胀而产生的。通过改变芯轴的旋转速度，使由多个区域组成的2D拉伸膜膨胀，形成纤维组织的梯度。由径向排列的纳米纤维组成的3D组件上的组成梯度通过滴落、扩散和交联来制备。与在具有较大孔径的组装件上培养的骨髓间充质干细胞（BMSC）相比，在具有较小孔径的3D生物相容性组装件上的骨髓间充质干细胞（BMSC）显示出显著更高的缺氧相关标志物表达和增强的软骨形成分化。碱性成纤维细胞生长因子（bFGF）梯度可以加速成纤维细胞从周围区域迁移到中心在体外伤口愈合模型。总之，具有孔径、纤维组织和信号分子含量梯度的3D微球组装体可用于工程化组织构建以用于组织修复，并构建仿生疾病模型以用于研究疾病生物学和筛选药物，特别是用于界面组织工程和建模。基于静电纺丝、气体发泡膨胀、涂覆、扩散、包封和交联，开发了一类具有结构和组成梯度的新型3D纳米组装体。这种在孔径、纤维组织和信号分子含量方面具有梯度的组装体可用于工程化组织构建体以用于组织修复，并构建仿生疾病模型以用于研究疾病生物学和筛选药物。
New methods are described for converting two-dimensional (2D) electrospun nanofiber membranes to three-dimensional (3D) hierarchical assemblies with structural and compositional gradients. Pore size gradients are generated by tuning the expansion of 2D membranes in different layers with incorporation of various amounts of a surfactant during the gas-foaming process. The gradient in fiber organizations is formed by expanding 2D nanofiber membranes composed of multiple regions collected by varying rotating speeds of mandrel. The compositional gradient on 3D assemblies consisting of radially aligned nanofibers is prepared by dripping, diffusion, and crosslinking. Bone mesenchymal stem cells (BMSCs) on the 3D nanofiber assemblies with smaller pore size show significantly higher expression of hypoxia related markers and enhanced chondrogenic differentiation compared to BMSCs cultured on the assemblies with larger pore size. The basic fibroblast growth factor (bFGF) gradient can accelerate fibroblast migration from the surrounding area to the center in an in vitro wound healing model. Taken together, 3D nanofiber assemblies with gradients in pore sizes, fiber organizations, and contents of signaling molecules can be used to engineer tissue constructs for tissue repair and build biomimetic disease models for studying disease biology and screening drugs, in particular, for interface tissue engineering and modeling. A new class of 3D nanofiber assemblies with structural and compositional gradients is developed based on electrospinning, gas-foaming expansion, coating, diffusion, encapsulation, and crosslinking. Such assemblies with gradients in pore sizes, fiber organizations, and contents of signaling molecules can be used to engineer tissue constructs for tissue repair and build biomimetic disease models for studying disease biology and screening drugs.
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影响因子：
9.7
作者：
Jiang J;Chen S;Wang H;Carlson MA;Gombart AF;Xie J
通讯作者：
Xie J
影响因子：
3.3
作者：
Sophia Fox AJ;Bedi A;Rodeo SA
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Rodeo SA
影响因子：
29.4
作者：
Lee, Wonjae;Frank, Curtis W.;Park, Jon
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Park, Jon
影响因子：
10.8
作者：
Chen, Shixuan;Wang, Hongjun;Xie, Jingwei
通讯作者：
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影响因子：
7.2
作者：
Iismaa SE;Kaidonis X;Nicks AM;Bogush N;Kikuchi K;Naqvi N;Harvey RP;Husain A;Graham RM
通讯作者：
Graham RM