Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution.
Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution.
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DOI：
10.1038/s41467-022-35123-6
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发表时间：
2022-12-03
影响因子：
16.6
通讯作者：
Huiskonen, Juha T.
中科院分区：
文献类型：
--
作者：
Kejzar, Nejc;Laanto, Elina;Rissanen, Ilona;Abrishami, Vahid;Selvaraj, Muniyandi;Moineau, Sylvain;Ravantti, Janne;Sundberg, Lotta-Riina;Huiskonen, Juha T.
病毒的起源仍然是一个悬而未决的问题。虽然缺乏可检测的序列相似性阻碍了分析远亲病毒，保守的衣壳蛋白结构的结构生物学研究促进了远距离进化关系的研究。在这里，我们描述了含脂质的ssDNA温和噬菌体Φ CjT 23，它感染黄杆菌属。我们报告了几种黄杆菌属菌株基因组中的Φ CjT 23样序列。通过低温电子显微镜确定的病毒体结构揭示了与病毒王国Bamford病毒的成员的相似性，Bamford病毒目前仅由双链DNA病毒组成，其主要衣壳蛋白由两个直立的β-三明治组成。Φ CjT 23的最小结构表明，该噬菌体可作为Bamford病毒中ssDNA和dsDNA病毒之间最后共同祖先的模型。Φ CjT 23和相关噬菌体FLiP都感染在几种环境中发现的黄杆菌属物种，表明这些类型的病毒具有全球分布和共同的进化起源。与相关的更复杂的病毒进行详细的比较，不仅扩大了我们对这组病毒的了解，而且还提供了对早期病毒进化的罕见一瞥。保守衣壳蛋白的结构生物学研究有助于病毒进化的研究。在这里，含脂质的ssDNA噬菌体Φ CjT 23的特征表明，这种噬菌体可能作为Bamfordviritus中ssDNA和dsDNA病毒之间最后共同祖先的模型。
The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution. Structural biology investigation of conserved capsid proteins facilitates the study of virus evolution. Here, characterization of the lipid-containing ssDNA bacteriophage ΦCjT23 suggests that this phage may serve as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae.
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影响因子：
3
作者：
de la Rosa-Trevin, J. M.;Quintana, A.;Carazo, J. M.
通讯作者：
Carazo, J. M.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
7.7
作者：
Bai, Xiao-chen;Rajendra, Eeson;Scheres, Sjors H. W.
通讯作者：
Scheres, Sjors H. W.
影响因子：
16.8
作者：
Huiskonen, JT;Kivelä, HM;Butcher, SJ
通讯作者：
Butcher, SJ
影响因子：
64.8
作者：
Abrescia, NGA;Cockburn, JJB;Bamford, JKH
通讯作者：
Bamford, JKH