Involvement of urinary bladder Connexin43 and the circadian clock in coordination of diurnal micturition rhythm.
Involvement of urinary bladder Connexin43 and the circadian clock in coordination of diurnal micturition rhythm.
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DOI：
10.1038/ncomms1812
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发表时间：
2012-05-01
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
儿童夜间遗尿症和老年人夜尿症是两种非常普遍的临床病症，其特征是睡眠阶段肾脏中的尿液产生率与膀胱中的储存率不匹配。在这里，我们使用一种自动记录小鼠排尿的新方法证明，膀胱间隙连接蛋白 connexin43 (Cx43) 是功能性膀胱容量的负调节因子。野生型小鼠的膀胱 Cx43 水平和功能能力显示出昼夜节律振荡，但在生物钟功能失调的 Cry-null 小鼠中，这种节律完全丧失。膀胱肌细胞有一个内部时钟，并表现出 Cx43 和间隙连接功能的振荡。时钟调节器 Rev-erbα 使用启动子的 Sp1 顺式元件上调作为 Sp1 辅助因子的 Cx43 转录。因此，Cx43 的昼夜节律振荡与生物钟相关，并有助于膀胱容量的昼夜变化，从而避免排尿干扰睡眠。
Nocturnal enuresis in children and nocturia in the elderly are two highly prevalent clinical conditions characterized by a mismatch between urine production rate in the kidneys and storage in the urinary bladder during the sleep phase. Here we demonstrate, using a novel method for automated recording of mouse micturition, that connexin43 (Cx43), a bladder gap junction protein, is a negative regulator of functional bladder capacity. Bladder Cx43 levels and functional capacity show circadian oscillations in wild-type mice, but such rhythms are completely lost in Cry-null mice having a dysfunctional biological clock. Bladder muscle cells have an internal clock, and show oscillations of Cx43 and gap junction function. A clock regulator, Rev-erbα, upregulates Cx43 transcription as a co-factor of Sp1 using Sp1 cis-elements of the promoter. Therefore, circadianoscillation of Cx43 is associated with the biological clock and contributes to diurnal changes in bladder capacity, which avoids disturbance of sleep by micturition.
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影响因子：
11.4
作者：
HAGEN, G;MULLER, S;SUSKE, G
通讯作者：
SUSKE, G
影响因子：
64.5
作者：
Balsalobre, A;Damiola, F;Schibler, U
通讯作者：
Schibler, U
DOI：
10.1083/jcb.90.2.521
发表时间：
1981-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fallon RF;Goodenough DA
通讯作者：
Goodenough DA
影响因子：
16.6
作者：
Doi, Masao;Ishida, Atsushi;Miyake, Akiko;Sato, Miho;Komatsu, Rie;Yamazaki, Fumiyoshi;Kimura, Ikuo;Tsuchiya, Soken;Kori, Hiroshi;Seo, Kazuyuki;Yamaguchi, Yoshiaki;Matsuo, Masahiro;Fustin, Jean-Michel;Tanaka, Rina;Santo, Yasuko;Yamada, Hiroyuki;Takahashi, Yukari;Araki, Michihiro;Nakao, Kazuki;Aizawa, Shinichi;Kobayashi, Masaki;Obrietan, Karl;Tsujimoto, Gozoh;Okamura, Hitoshi
通讯作者：
Okamura, Hitoshi
影响因子：
3.5
作者：
BOIVIN, DB;DUFFY, JF;CZEISLER, CA
通讯作者：
CZEISLER, CA