Function and evolution of two forms of SecDF homologs in Streptomyces coelicolor.
Function and evolution of two forms of SecDF homologs in Streptomyces coelicolor.
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DOI：
10.1371/journal.pone.0105237
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Zhou Z;Li Y;Sun N;Sun Z;Lv L;Wang Y;Shen L;Li YQ
一般分泌（Sec）途径在细菌蛋白质输出中起着重要作用，辅助组分SecDF已被证明可以提高运输效率。天蓝色链霉菌基因组的检查揭示了两种不同形式的secDF同源基因的意外存在：一种是融合形式（secDF），另一种是分离形式（secD和secF）。然而，两个SecDF同源物在S. coelicolor尚未确定。secDF同源物的转录分析表明，这些基因组成型表达。而在S.天蓝色。删除S中的secDF或/和secD/secF。coelicolor确实导致木聚糖酶A和淀粉酶C的分泌效率降低，这表明它们可能在Sec依赖的转运途径中具有冗余功能。此外，我们的研究结果还表明，SecD/SecF发挥更突出的作用比SecDF的蛋白质转运。进化分析表明，链霉菌中融合和分离的SecDF同源物可能具有不同的进化祖先。SecD/SecF可能来源于链霉菌祖先已有组分的垂直传递。然而，SecDF可能通过水平基因转移来自细菌祖先。或者，SecDF可能是通过额外的基因复制和融合事件产生的，这也是合理的。获得第二个拷贝可以通过增强蛋白质转运能力而赋予链霉菌选择性益处。两者合计，我们的研究结果提供了新的见解的潜在的生物学功能和进化方面的原核SecDF复合物。
The general secretion (Sec) pathway plays a prominent role in bacterial protein export, and the accessory component SecDF has been shown to improve transportation efficiency. Inspection of Streptomyces coelicolor genome reveals the unexpected presence of two different forms of secDF homologous genes: one in fused form (secDF) and the other in separated form (secD and secF). However, the functional role of two SecDF homologs in S. coelicolor has not yet been determined. Transcriptional analysis of secDF homologs reveals that these genes are constitutively expressed. However, the transcript levels of secD and secF are much higher than that of secDF in S. coelicolor. Deletion of secDF or/and secD/secF in S. coelicolor did result in reduced secretion efficiency of Xylanase A and Amylase C, suggesting that they may have redundant functions for Sec-dependent translocation pathway. Moreover, our results also indicate that SecD/SecF plays a more prominent role than SecDF in protein translocation. Evolutionary analysis suggests that the fused and separated SecDF homologs in Streptomyces may have disparate evolutionary ancestries. SecD/SecF may be originated from vertical transmission of existing components from ancestor of Streptomyces species. However, SecDF may be derived from bacterial ancestors through horizontal gene transfer. Alternately, it is also plausible that SecDF may have arisen through additional gene duplication and fusion events. The acquisition of a second copy may confer a selective benefit to Streptomyces by enhancing protein transport capacity. Taken together, our results provide new insights into the potential biological function and evolutionary aspects of the prokaryotic SecDF complex.
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发表时间：
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