Evidence from the stop‐EGFP mouse supports a niche‐sharing model of epidermal proliferative units
Evidence from the stop‐EGFP mouse supports a niche‐sharing model of epidermal proliferative units
复制标题
来自 stop-EGFP 小鼠的证据支持表皮增殖单位的生态位共享模型
DOI：
10.1111/j.1600-0625.2005.00366.x
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发表时间：
2005
影响因子：
3.6
通讯作者：
B. Rannala
中科院分区：
文献类型：
--
作者：
S. Ro;B. Rannala
摘要：经典的表皮增殖单位（EPU）模型假设每个EPU由角质层细胞和其正下方的表皮细胞组成，并由单位内的单个干细胞维持。利用停止增强绿色荧光蛋白（stop - EGFP）转基因小鼠系统，我们先前发现表皮干细胞克隆谱系可以产生多个相邻的角质层细胞（即表皮细胞属于多个相邻的EPU），这与经典的EPU模型相矛盾。我们早期研究的一个可能问题是使用N -乙基- N -亚硝基脲（ENU）产生克隆分析的突变。这可能会改变表皮组织的正常环境，并可能导致干细胞克隆谱系的人工扩增。在这项研究中，我们使用未经处理的停止EGFP小鼠并依靠自发突变来产生克隆细胞系，重复了我们早期的发现。我们提出了一个替代经典EPU模型来解释表皮增殖的动态性质。我们的epu生态位共享模型允许表皮细胞在epu之间水平迁移，从而使多个干细胞协同维持更大的增殖空间。
Abstract: The classical model of epidermal proliferative units (EPUs) postulates that each EPU is composed of a single column of corneocytes plus epidermal cells directly below the column and is maintained by a single stem cell within the unit. Using the stop‐enhanced green fluorescent protein (stop‐EGFP) transgenic mouse system, we previously showed epidermal stem cell clonal lineages could produce multiple adjacent corneocytes (i.e. epidermal cells belonging to multiple adjacent EPUs), contradicting the classical EPU model. One possible problem with our earlier study was that N‐ethyl‐N‐nitrosourea (ENU) was used to generate mutations for clonal analysis. This could alter the normal environment of the epidermal tissue and might lead to an artificial expansion of stem cell clonal lineages. In this study, we replicate our earlier findings using untreated stop‐EGFP mice and relying on spontaneous mutations to generate clonal cell lineages. We propose an alternative to the classical EPU model to explain the dynamic nature of epidermal proliferation. Our niche‐sharing model of EPUs allows epidermal cells to horizontally migrate among EPUs, so that multiple stem cells cooperatively maintain a larger proliferative compartment.
影响因子：
6.5
作者：
Mackenzie, IC
通讯作者：
Mackenzie, IC
影响因子：
6.5
作者：
Ghazizadeh, S;Taichman, LB
通讯作者：
Taichman, LB