Low-Cost Microplate Reader with 3D Printed Parts for under 500 USD.
Low-Cost Microplate Reader with 3D Printed Parts for under 500 USD.
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DOI：
10.3390/s22093242
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发表时间：
2022-04-23
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影响因子：
--
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中科院分区：
文献类型：
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设计、建造并测试了一台用于吸收光谱分析的96孔酶标仪，总成本约为。500美元。器械成本的降低代表了本手稿的主要技术贡献，因为成本比之前的报告降低了7倍。该设备能够达到3σ检测限的ca。0.01对于中可见波长范围，在60秒测量内的吸光度单位（Au）。部件可以在市场上买到，也可以从平面图中3D打印出来。分析波长可以通过使用各种摄影或戏剧滤光片在整个可见光区改变。该功能允许孔板读数器用于典型的实验室测定，例如通过600 nm处的光密度（OD）或450 nm处的酶联免疫吸附测定（ELISA）进行细胞群体估计。这份手稿报告的动机和构建设备的过程中，列出所需的部件，提出数据证明设备的功能，并提供了学者的社区计划，以重现工作。该设备可以在缺乏足够资源购买商业可用选项的实验室中复制，这一结果有助于增强个人权能和科学探究的公平性。
A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. 500 USD. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. The device was able to achieve 3σ limits of detection of ca. 0.01 absorbance units (AU) over a 60 second measurement for the mid-visible wavelength range. Component parts are either commercially available, or 3D printed from plans. Analysis wavelength can be altered throughout the visible region through use of various photographic or theatrical filters. This feature allows the well plate reader to be used for typical laboratory assays such as cell population estimation by optical density (OD) at 600 nm, or enzyme-linked immunosorbent assays (ELISA) at 450 nm. This manuscript reports on the motivation and process of constructing the device, lists required parts, presents data demonstrating device function, and provides the community of scholars with plans to reproduce the work. The device can be reproduced in laboratories lacking sufficient resources to purchase commercially available options and this outcome contributes towards empowerment of individuals and equity of scientific enquiry.
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影响因子：
9.8
作者：
Baden T;Chagas AM;Gage GJ;Marzullo TC;Prieto-Godino LL;Euler T
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