mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues.
mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues.
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DOI：
10.1111/j.1365-2141.2010.08398.x
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发表时间：
2011-02
影响因子：
6.5
通讯作者：
Felix CA
中科院分区：
文献类型：
--
作者：
Robinson BW;Germano G;Song Y;Abrams J;Scott M;Guariento I;Tiso N;Argenton F;Basso G;Rhodes J;Kanki JP;Look AT;Balice-Gordon RJ;Felix CA
Infant leukaemia is an embryonal disease in which the underlying MLL translocations initiate in utero. Zebrafish offer unique potential to understand how MLL impacts haematopoiesis from the earliest embryonic timepoints and how translocations cause leukaemia as an embryonal process. In this study, a zebrafish mll cDNA syntenic to human MLL spanning the 5’ to 3’UTRs, was cloned from embryos, and mll expression was characterized over the zebrafish lifespan. The protein encoded by the 35-exon ORF exhibited 46.4% overall identity to human MLL and 68–100% conservation in functional domains (AT-hooks, SNL, CXXC, PHD, bromodomain, FYRN, taspase1 sites, FYRC, SET). Maternally supplied transcripts were detected at 0–2 hpf. Strong ubiquitous early zygotic expression progressed to a cephalo-caudal gradient during later embryogenesis. mll was expressed in the intermediate cell mass (ICM) where primitive erythrocytes are produced and in the kidney where definitive haematopoiesis occurs in adults. mll exhibits high cross species conservation, is developmentally regulated in haematopoietic and other tissues and is expressed from the earliest embryonic timepoints throughout the zebrafish lifespan. Haematopoietic tissue expression validates using zebrafish for MLL haematopoiesis and leukaemia models.
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影响因子：
20.3
作者：
Felix, CA;Kim, CS;Rappaport, EF
通讯作者：
Rappaport, EF
影响因子：
30.8
作者：
DJABALI, M;SELLERI, L;EVANS, GA
通讯作者：
EVANS, GA
影响因子：
20.3
作者：
Hess, JL;Yu, BD;Korsmeyer, SJ
通讯作者：
Korsmeyer, SJ
影响因子：
14.9
作者：
Barreau C;Paillard L;Osborne HB
通讯作者：
Osborne HB
影响因子：
4.5
作者：
Hu, J;Lutz, CS;Tian, B
通讯作者：
Tian, B