Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study.
Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study.
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DOI：
10.1371/journal.pone.0056828
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Waitumbi JN
中科院分区：
文献类型：
--
作者：
Bashir IM;Otsyula N;Awinda G;Spring M;Schneider P;Waitumbi JN
与专业疟疾显微镜相比，疟疾生物标志物如恶性疟原虫富组氨酸蛋白-2（PfHRP-2）和PCR提供了用于量化疟疾寄生虫感染的上级分析灵敏度和特异性。本研究报告了疟疾疫苗第一阶段试验期间采用不同诊断方法的寄生虫流行率、患病访视寄生虫密度和物种组成。在计划访视（n = 298）或患病访视（n = 38）期间，从参与112天疫苗试验的30名成人中采集用于显微镜检查、PfHRP-2和疟原虫乳酸脱氢酶（pLDH）ELISA和真实的实时定量PCR（qPCR）的血样。 这四种方法用于评估寄生虫患病率，以及临床发作患者42天内的寄生虫密度。在计划访视期间，qPCR（39.9%，N = 119）和PfHRP-2 ELISA（36.9%，N = 110）检测到的寄生虫患病率高于pLDH ELISA（16.8%，N = 50），所有方法均高于显微镜检查（13.4%，N = 40）。 所有显微镜下检测到的感染均含有恶性疟原虫，为单一感染（95%）或三日疟原虫（5%）。通过qPCR，119例感染中有102例为物种鉴定。恶性疟原虫以单一感染为主（71.6%），其次为三日疟原虫（8.8%）、卵形疟原虫（4.9%）或两者兼有（3.9%）。三日疟原虫（6.9%）和卵形疟原虫（1.0%）也作为合并感染（2.9%）发生。正如预期的那样，在患病访视期间检测到较高的患病率，患病率为65.8%（qPCR）、60.5%（PfHRP-2 ELISA）、21.1%（pLDH ELISA）和31.6%（显微镜检查）。PfHRP-2显示在临床发作时达到高峰的生物量积累（1813±3410 ng/mL SD）。 可能需要PfHRP-2 ELISA和qPCR来准确定量疟原虫负荷。此外，qPCR改善了寄生虫物种形成，而PfHRP-2 ELISA是恶性疟原虫引起的临床疾病的潜在预测因子。ClinicalTrials.gov NCT00666380
Compared to expert malaria microscopy, malaria biomarkers such as Plasmodium falciparum histidine rich protein-2 (PfHRP-2), and PCR provide superior analytical sensitivity and specificity for quantifying malaria parasites infections. This study reports on parasite prevalence, sick visits parasite density and species composition by different diagnostic methods during a phase-I malaria vaccine trial. Blood samples for microscopy, PfHRP-2 and Plasmodium lactate dehydrogenase (pLDH) ELISAs and real time quantitative PCR (qPCR) were collected during scheduled (n = 298) or sick visits (n = 38) from 30 adults participating in a 112-day vaccine trial. The four methods were used to assess parasite prevalence, as well as parasite density over a 42-day period for patients with clinical episodes. During scheduled visits, qPCR (39.9%, N = 119) and PfHRP-2 ELISA (36.9%, N = 110) detected higher parasite prevalence than pLDH ELISA (16.8%, N = 50) and all methods were more sensitive than microscopy (13.4%, N = 40). All microscopically detected infections contained P. falciparum, as mono-infections (95%) or with P. malariae (5%). By qPCR, 102/119 infections were speciated. P. falciparum predominated either as monoinfections (71.6%), with P. malariae (8.8%), P. ovale (4.9%) or both (3.9%). P. malariae (6.9%) and P. ovale (1.0%) also occurred as co-infections (2.9%). As expected, higher prevalences were detected during sick visits, with prevalences of 65.8% (qPCR), 60.5% (PfHRP-2 ELISA), 21.1% (pLDH ELISA) and 31.6% (microscopy). PfHRP-2 showed biomass build-up that climaxed (1813±3410 ng/mL SD) at clinical episodes. PfHRP-2 ELISA and qPCR may be needed for accurately quantifying the malaria parasite burden. In addition, qPCR improves parasite speciation, whilst PfHRP-2 ELISA is a potential predictor for clinical disease caused by P. falciparum. ClinicalTrials.gov NCT00666380
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影响因子：
9.4
作者：
Kamau, Edwin;Tolbert, LaDonna S.;Ockenhouse, Christian F.
通讯作者：
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DOI：
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发表时间：
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