Organization of sympathetic innervation of interscapular brown adipose tissue in the mouse.
Organization of sympathetic innervation of interscapular brown adipose tissue in the mouse.
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DOI：
10.1002/cne.25281
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发表时间：
2022-06
影响因子：
2.5
通讯作者：
Munzberg, Heike
中科院分区：
文献类型：
--
作者：
Huesing, Clara;Zhang, Rui;Gummadi, Sanjeev;Lee, Nathan;Qualls-Creekmore, Emily;Yu, Sangho;Morrison, Christopher D.;Burk, David;Berthoud, Hans Rudolf;Neuhuber, Winfried;Munzberg, Heike
关键词：
肩胛间棕色脂肪组织（iBAT）受交感神经控制，最近的研究强调了传出交感神经和传入感觉或体液反馈系统对调节脂肪组织功能和整体代谢健康的重要性。然而，由于缺乏解剖学和精细结构的细节，对小鼠交感神经系统的功能研究是有限的。在这里，我们使用表达酪氨酸羟化酶的神经元的报告小鼠（TH：番茄小鼠）、iDISCO 组织清除、共聚焦、光片和电子显微镜来阐明：a) iBAT 通过背支（而不是经常提到的肋间神经）接收交感神经输入； b) 背支 T1–T5 对应于交感链神经节的节后输入（星状/T1–T5）； c) 背支充当交感神经轴突的导管，交感神经轴突在更细的神经束中分支进入 iBAT； d) 轴突静脉曲张显示出棕色（密集神经支配）与白色（稀疏神经支配）脂肪细胞的强烈神经支配差异，这些脂肪细胞围绕小鼠的核心 iBAT 并在人类脂肪组织中混合，e) 轴突静脉曲张可以与棕色脂肪细胞形成神经脂肪细胞连接。综上所述，我们证明交感 iBAT 神经支配是由特定的神经和末梢结构组织的，可以通过手术和基因方法进行神经调节。交感神经 iBAT 神经支配的示意图模型。肩胛间棕色脂肪组织 (iBAT) 神经支配通过背支而不是肋间神经（又名腹支）到达目标。背支 1-5 代表交感链神经节输入（星状神经节到 T5）。细神经从每个背支分支进入 iBAT 进行神经支配，而背支继续支配其他背部结构，如皮肤。棕色脂肪细胞（BA）受到形成神经脂肪细胞连接的轴突静脉曲张的密集神经支配，而白色脂肪细胞在小鼠和人类脂肪组织中表现出稀疏的神经支配。核心 iBAT 神经支配密度在冷暴露后保持不变，但白色脂肪组织主要在米色岛内增加。
The interscapular brown adipose tissue (iBAT) is under sympathetic control, and recent studies emphasized the importance of efferent sympathetic and afferent sensory or humoral feedback systems to regulate adipose tissue function and overall metabolic health. However, functional studies of the sympathetic nervous system in the mouse are limited, because details of anatomy and fine structure are lacking. Here, we used reporter mice for tyrosine hydroxylase expressing neurons (TH:tomato mice), iDISCO tissue clearance, confocal, light sheet, and electron microscopy to clarify that a) iBAT receives sympathetic input via dorsal rami (instead of often cited intercostal nerves); b) dorsal rami T1–T5 correspond to the post-ganglionic input from sympathetic chain ganglia (stellate/T1–T5); c) dorsal rami serve as conduits for sympathetic axons that branch off in finer nerve bundles to enter iBAT; d) axonal varicosities show strong differential innervation of brown (dense innervation) versus white (sparse innervation) adipocytes, that surround the core iBAT in the mouse and are intermingled in human adipose tissues, e) axonal varicosities can form neuro-adipocyte junctions with brown adipocytes. Taken together, we demonstrate that sympathetic iBAT innervation is organized by specific nerves and terminal structures that can be surgically and genetically accessed for neuromodulatory purposes. Schematic model for sympathetic iBAT innervation. The interscapular brown adipose tissue (iBAT) innervation reaches their targets via dorsal rami, not intercostal nerves (aka ventral rami). Dorsal rami 1–5 represent sympathetic chain ganglia inputs (stellate ganglion to T5). Fine nerves branch off each dorsal ramus to enter the iBAT for innervation, while dorsal rami continue to innervate additional dorsal structures like the skin. Brown adipocytes (BA) are densely innervated by axonal varicosities that form neuro-adipocyte junctions, while white adipocytes show sparse innervation in mouse and human adipose tissues. Core iBAT innervation density remains unchanged by cold exposure, but increases in white adiopose tissue mainly within beige islands.
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影响因子：
4.6
作者：
Jeong JH;Chang JS;Jo YH
通讯作者：
Jo YH
DOI：
10.1016/0165-1838(94)00171-f
发表时间：
1995-06-25
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
BARON, R;JANIG, W;WITH, H
通讯作者：
WITH, H
DOI：
10.1007/bf00324749
发表时间：
1968-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
BARGMANN, W;VONHEHN, G;LINDNER, E
通讯作者：
LINDNER, E
DOI：
10.1038/ijosup.2015.9
发表时间：
2015-08-01
期刊：
International journal of obesity supplements
影响因子：
--
作者：
Bartness, T J;Ryu, V
通讯作者：
Ryu, V
影响因子：
4.8
作者：
Grujic, D;Susulic, VS;Lowell, BB
通讯作者：
Lowell, BB