The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli.
The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli.
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DOI：
10.1111/j.1365-2958.2011.07539.x
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发表时间：
2011-03
影响因子：
3.6
通讯作者：
Silhavy TJ
中科院分区：
文献类型：
--
作者：
Cowles CE;Li Y;Semmelhack MF;Cristea IM;Silhavy TJ
脂蛋白LPP是大肠杆菌中数量最丰富的蛋白质，经过40多年的研究，自发现以来一直是细菌脂蛋白的聚集性。它以两种截然不同的形式存在：“结合形式”和“自由形式”，前者共价结合到细胞的肽聚糖层上，后者则不是。虽然已知结合形式LPP的羧基末端位于周质中，但自由形式LPP的确切位置一直没有确定。几十年来，人们普遍认为自由形式的LPP与束缚形式联系在一起。在这项工作中，我们证明了LPP的自由形式和结合形式并不是主要相互关联的，而是在不同的亚细胞位置上发现的。我们的结果表明，自由形式的LPP跨越外膜，表面暴露，而结合形式的LPP驻留在周质中。因此，LPP代表了一个能够占据不同亚细胞位置的单一脂蛋白的新例子，并挑战了假设LPP的游离型和结合型相互关联的模型。
The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a “bound-form”, which is covalently bound to the cell’s peptidoglycan layer, and a “free-form”, which is not. Although it is known that the carboxyl-terminus of bound-form Lpp is located in the periplasm, the precise location of free-form Lpp has never been determined. For decades, it has been widely assumed that free-form Lpp is associated with bound-form. In this work, we show that the free and bound forms of Lpp are not largely associated with each other, but are found in distinct subcellular locations. Our results indicate that free-form Lpp spans the outer membrane and is surface-exposed, whereas bound-form Lpp resides in the periplasm. Thus, Lpp represents a novel example of a single lipoprotein that is able to occupy distinct subcellular locations, and challenges models in which the free and bound forms of Lpp are assumed to be associated with each other.
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DOI：
10.1016/j.jasms.2009.08.026
发表时间：
2010-01
影响因子：
3.2
作者：
Luo Y;Li T;Yu F;Kramer T;Cristea IM
通讯作者：
Cristea IM
DOI：
10.1073/pnas.77.8.4592
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHEN, R;SCHMIDMAYR, W;HENNING, U
通讯作者：
HENNING, U
影响因子：
3.2
作者：
COLE, ST;CHENSCHMEISSER, U;HENNING, U
通讯作者：
HENNING, U
影响因子：
3.6
作者：
Leuzzi, R;Serino, L;Pizza, M
通讯作者：
Pizza, M
DOI：
10.1111/j.1432-1033.1970.tb00936.x
发表时间：
1970-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BRAUN, V;SIEGLIN, U
通讯作者：
SIEGLIN, U