Comparison of pathogen detection consistency between metagenomic next-generation sequencing and blood culture in patients with suspected bloodstream infection.
Comparison of pathogen detection consistency between metagenomic next-generation sequencing and blood culture in patients with suspected bloodstream infection.
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DOI：
10.1038/s41598-023-36681-5
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发表时间：
2023-06-10
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
元基因组下一代测序技术(MNGS)的应用已逐渐被临床实践人员所接受。然而，很少有研究将其与疑似血液感染患者的血液培养进行比较。这项研究的目的是比较这两种检测方法对疑似血流感染患者的病原微生物的检测。我们对2020年1月至2022年6月瑞金医院急诊科就诊的发热、寒战、抗生素使用3天以上、疑似血流感染、入院的患者进行了回顾性研究。所有患者均在同一天抽血进行血MNGS和血培养。在抽血当天收集临床和实验室参数。比较两种方法对病原微生物的检测结果。对于这两种检测方法，分别分析了血液感染患者的危险因素和住院死亡率。在99例患者中，血MNGS中病原菌的检出率显著高于血培养。在所有阳性细菌和真菌检测结果中，血mNGS与血培养符合率仅为12.00%。C反应蛋白水平与血MNGS检测的菌血症、菌血症、病毒血症有关。在血培养阳性的患者中未发现明确的危险因素。在危重患者中，这两种测试都未能改善患者的预后。在疑似血液感染的患者中，mNGS还不能完全替代血培养。
The application of metagenomic next-generation sequencing (mNGS) has gradually been carried out by clinical practitioner. However, few studies have compared it with blood cultures in patients suffering from suspected bloodstream infections. The purpose of this study was to compare the detection of pathogenic microorganisms by these two assays in patients with suspected bloodstream infection. We retrospectively studied patients with fever, chills, antibiotic use for more than 3 days, suspected bloodstream infection, and admission to the emergency department of Ruijin Hospital from January 2020 to June 2022. All patients had blood drawn on the same day for blood mNGS and blood cultures. Clinical and laboratory parameters were collected on the day blood was drawn. The detection of pathogenic microorganisms by the two methods was compared. Risk factors and in-hospital mortality in patients with bloodstream infections were analysed separately for these two assays. In all 99 patients, the pathogenic microorganisms detection rate in blood mNGS was significantly higher than that in blood culture. Blood mNGS was consistent with blood culture in only 12.00% of all positive bacterial and fungal test results. The level of CRP is related to bacteraemia, fungaemia and viraemia detected by blood mNGS. No clear risk factors could be found in patients with a positive blood culture. In critically ill patients, both tests failed to improve patient outcomes. In patients with suspected bloodstream infection, mNGS is not yet a complete replacement for blood cultures.
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危重患者血培养阳性的预测因素：回顾性评估。
影响因子：
1.9
作者：
Previsdomini M;Gini M;Cerutti B;Dolina M;Perren A
通讯作者：
Perren A
影响因子：
5.7
作者：
Lecuit M;Eloit M
通讯作者：
Eloit M
DOI：
10.1186/s13054-021-03592-8
发表时间：
2021-05-08
期刊：
Critical care (London, England)
影响因子：
--
作者：
Li Y;Guo J;Yang H;Li H;Shen Y;Zhang D
通讯作者：
Zhang D
影响因子：
39.2
作者：
Cheng, Matthew P.;Stenstrom, Robert;Osati, Farzaneh
通讯作者：
Osati, Farzaneh
影响因子：
11.8
作者：
Kontula KSK;Skogberg K;Ollgren J;Järvinen A;Lyytikäinen O
通讯作者：
Lyytikäinen O