Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis.
Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis.
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DOI：
10.1186/s40199-015-0117-6
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发表时间：
2015-07-04
期刊：
影响因子：
--
通讯作者：
Cho H
中科院分区：
文献类型：
--
作者：
Lee HH;Lee S;Lee K;Shin YS;Kang H;Cho H
蛹虫草在亚洲国家被用作传统药物已有很长时间。据报道，不同类型的虫草提取物具有各种药理活性，包括抗癌作用。我们研究了蛹虫草乙醇提取物对人结直肠癌细胞系RKO的抑制作用。用不同浓度的富含核苷的蛹虫草乙醇提取物处理RKO细胞48 h，并使用CCK-8测定法测量细胞毒性。然后，异种移植Balb/c裸鼠注射RKO细胞，随后每天口服蛹虫草乙醇提取物3周，以检查对肿瘤生长的抑制作用。最后，采用流式细胞仪检测蛹虫草对细胞周期和凋亡的影响。Western blot检测p53、caspase 9、caspase-3、PARP、Bim、Bax、巴克和Bad的表达。RKO细胞对蛹虫草乙醇提取物（CME）高度敏感，蛹虫草处理后RKO细胞源性肿瘤的生长明显延迟。蛹虫草可诱导细胞周期阻滞于G2/M期（未处理组; 20.5%，CME 100 μg/ml; 61.67%，CME 300 μg/ml; 66.33%），并增加早期凋亡（未处理组; 1.01%，CME 100 μg/ml; 8.48%，CME 300 μg/ml; 18.07%）。蛹虫草处理后p53、切割的caspase 9、切割的caspase-3、切割的PARP、Bim、巴克和Bad的表达上调。蛹虫草乙醇提取物对人大肠癌RKO细胞具有较强的细胞毒作用，并能抑制异种移植瘤的生长。蛹虫草的抗肿瘤作用与诱导细胞周期阻滞和细胞凋亡有关。
Cordyceps militaris has been used as a traditional medicine in Asian countries for a long time. Different types of Cordyceps extract were reported to have various pharmacological activities including an anti-cancer effect. We investigated the inhibitory effect of Cordyceps militaris ethanol extract on a human colorectal cancer-derived cell line, RKO. RKO cells were treated with various concentrations of nucleosides-enriched ethanol extract of Cordyceps militaris for 48 h and cytotoxicity was measured using a CCK-8 assay. Then, xenograft Balb/c nude mice were injected with RKO cells and subsequently orally administered with ethanol extract of Cordyceps militaris every day for 3 weeks to examine the inhibitory effect on tumor growth. Lastly, the effect of Cordyceps militaris on cell cycle as well as apoptosis was measured using flow cytometry. Also, the expression of p53, caspase 9, cleaved caspase-3, cleaved PARP, Bim, Bax, Bak, and Bad were detected using western blot assay. RKO cells were highly susceptible to the ethanol extract of Cordyceps militaris (CME) and the growth of RKO cells-derived tumor was significantly delayed by the treatment of Cordyceps militaris. Cordyceps militaris induced cell cycle arrest in G2/M phase (untreated; 20.5 %, CME 100 μg/ml; 61.67 %, CME 300 μg/ml; 66.33 %) and increased early apoptosis (untreated; 1.01 %, CME 100 μg/ml; 8.48 %, CME 300 μg/ml; 18.07 %). The expression of p53, cleaved caspase 9, cleaved caspase-3, cleaved PARP, Bim, Bak, and Bad were upregulated by the treatment of Cordyceps militaris. Ethanol extract of Cordyceps militaris was highly cytotoxic to human colorectal carcinoma RKO cells and inhibited the growth of tumor in xenograft model. The anti-tumor effect of Cordyceps militaris was associated with an induction of cell cycle arrest and mitochondrial-mediated apoptosis.
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影响因子：
3
作者：
Son, Myoungki;Lee, Minjung;Kang, Hyojeung
通讯作者：
Kang, Hyojeung
影响因子：
4.8
作者：
Boulares, AH;Yakovlev, AG;Smulson, M
通讯作者：
Smulson, M
影响因子：
5.4
作者：
Won, SY;Park, EH
通讯作者：
Park, EH
影响因子：
4.3
作者：
Lee, Seung Yuan;Debnath, Trishna;Lim, Beong Ou
通讯作者：
Lim, Beong Ou
DOI：
10.1615/intjmedmushr.v16.i3.30
发表时间：
2014-01-01
影响因子：
1.2
作者：
Kunjadia, Prashant D.;Nagee, Anju;Dave, Gaurav S.
通讯作者：
Dave, Gaurav S.