The 85-kilodalton phosphoprotein (pp85) of human herpesvirus 7 is encoded by open reading frame U14 and localizes to a tegument substructure in virion particles
The 85-kilodalton phosphoprotein (pp85) of human herpesvirus 7 is encoded by open reading frame U14 and localizes to a tegument substructure in virion particles
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人类疱疹病毒 7 型的 85 千道尔顿磷蛋白 (pp85) 由开放阅读框 U14 编码，定位于病毒粒子的外皮亚结构
作者：
Alessandra Stefan;P. Secchiero;T. Baechi;W. Kempf;G. Campadelli
在感染人类疱疹病毒7号（HHV-7）的细胞中存在一个抗原相关蛋白家族，被称为磷酸蛋白85 (pp85)，它包括一个蛋白复合体，其中85- kda蛋白被磷酸化。pp85是人类对HHV-7感染反应的主要决定因素(L. Foà-Tomasi， E. avitile， L. Ke, and G. Campadelli-Fiume， J. Gen. Virol. 75:2719- 2727,1994； L. Foà-Tomasi， M. P. Fiorilli， E. Avitabile, and G. Campadelli-Fiume， J. Gen. Virol. 77:511- 518,1996； J. B. Black等，临床。成岩作用。实验室。免疫学杂志，3:79-83,1996)。利用单克隆抗体（MAb） 5E1免疫筛选来自HHV-7感染细胞的cDNA文库，将编码pp85的基因定位到HHV-7基因组的U14开放阅读框上。在免疫印迹实验中，原核表达的含有U14开放阅读框的融合蛋白与MAb 5E1反应。通过免疫电镜研究确定了pp85的功能作用。对感染hhv -7的脐带血单个核细胞冷冻切片进行高分辨率免疫金标记，可将MAb 5E1的反应性定位在病毒粒子外膜表面。这一发现表明，U14基因的产物pp85是HHV-7被膜的一个组成部分，并表明HHV-7被膜不是一个均匀的结构，而是由亚结构组成，包括最外层含有pp85。目前的研究结果，连同先前报道的MAb 5E1的特性，包括其与福尔马林固定石蜡包埋样品的反应能力，使该抗体成为人类HHV-7感染的病原学研究的一个有用的特定工具，并为pp85进一步发展为特异性重组诊断试剂提供了基础。
A family of antigenically related proteins present in cells infected with human herpesvirus 7 (HHV-7), designated phosphoprotein 85 (pp85), comprises a complex of proteins, of which the 85-kDa species is phosphorylated. pp85 is a major determinant of human response to HHV-7 infection (L. Foà-Tomasi, E. Avitabile, L. Ke, and G. Campadelli-Fiume, J. Gen. Virol. 75:2719-2727, 1994; L. Foà-Tomasi, M. P. Fiorilli, E. Avitabile, and G. Campadelli-Fiume, J. Gen. Virol. 77:511-518, 1996; J. B. Black et al., Clin. Diagn. Lab. Immunol. 3:79-83, 1996). By immunoscreening of a cDNA library from HHV-7-infected cells with monoclonal antibody (MAb) 5E1, directed to the proteins of the pp85 complex, we mapped the gene encoding pp85 to the U14 open reading frame of the HHV-7 genome. A prokaryotically expressed fusion protein containing the U14 open reading frame reacted with MAb 5E1 in an immunoblot assay. A functional role for pp85 was defined by immunoelectron microscopy studies. Immunogold labeling of cryosections of HHV-7-infected cord blood mononuclear cells at high resolution localized the reactivity of MAb 5E1 to the outer surface of the virion tegument. This finding demonstrates that pp85, the product of the U14 gene, is a component of the HHV-7 tegument and suggests that the HHV-7 tegument is not a homogeneous structure but rather is composed of substructures, including an outermost layer containing pp85. The present findings, together with previously reported properties of MAb 5E1, including its ability to react with formalin-fixed paraffin-embedded samples, make this antibody a specific tool useful for etiopathogenetic studies of HHV-7 infection in humans and provide the basis for further development of pp85 into a specific recombinant diagnostic reagent.
人疱疹病毒7株JI基因组的克隆、限制性内切酶作图和部分序列分析。
DOI：
10.1099/0022-1317-77-8-1901
发表时间：
1996
期刊：
The Journal of general virology
影响因子：
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作者：
Ruvolo,VR;Berneman,Z;Secchiero,P;Nicholas,J
通讯作者：
Nicholas,J