Cloning, restriction endonuclease mapping and partial sequence analysis of the genome of human herpesvirus 7 strain JI.
Cloning, restriction endonuclease mapping and partial sequence analysis of the genome of human herpesvirus 7 strain JI.
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人疱疹病毒7株JI基因组的克隆、限制性内切酶作图和部分序列分析。
DOI：
10.1099/0022-1317-77-8-1901
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Nicholas,J
中科院分区：
文献类型：
--
作者：
Ruvolo,VR;Berneman,Z;Secchiero,P;Nicholas,J
人类疱疹病毒7型(HHV-7)是最近分离到的一种疱疹病毒，它与人巨细胞病毒和人类疱疹病毒6有亲缘关系，是β疱疹病毒亚群中的一员。本文报道了HHV-7株JI株DNA的克隆、限制性内切酶图谱和部分序列分析。从感染的SupT1细胞上清液中提取病毒颗粒，用蛋白酶K处理-苯酚抽提法提取DNA，并在脉冲场凝胶上纯化和分离全长病毒DNA。对高纯度的材料进行以下处理：(I)超声和末端修复，产生随机剪切的短片段，克隆到M13mp18-SMAL载体；(Ii)用EcoRI切割，克隆到EcoRI-CutλZAPII或λDASHII载体；(Iii)BamHI切割，克隆到BamHI-CutλZAP-Express或λDASHII载体。对M13克隆进行部分核苷酸测序，然后检测开放阅读框及其翻译，可以通过数据库的FASTA搜索来鉴定同源物。以相关的M13克隆为探针分离相应的λ噬菌体克隆，根据推测的HHV7和HHV6的遗传共线性，初步将其定位到基因组上。基因组“行走”在EcoRI和BamHIλ基因组文库之间，使重叠的相邻克隆得以识别和定位。这些克隆中的每一个都被分析到了BamHI、EcoRI、Sall、Smal和Xhol限制性内切酶位点，以提供整个基因组的完整内切酶图谱。
Human herpesvirus 7 (HHV-7) is a recently isolated herpesvirus that has been shown to be related to human cytomegalovirus and human herpesvirus 6 and to be a member of the betaherpesvirus subgroup. Here we report the cloning, restriction endonuclease mapping and partial sequence analysis of HHV-7 strain JI DNA. Virus particles were obtained from the supernatant of infected SupT1 cells, the DNA isolated by proteinase K treatment-phenol extraction, and full-length viral DNA was purified and isolated on a pulsed-field gel. Aliquots of this highly purified material were treated in the following ways: (i) sonicated and end-repaired to create short randomly sheared fragments for cloning into M13mp18-Smal vector DNA; (ii) cut withEcoRI for cloning intoEcoRI-cut λZAPII or λDASHII vectors; (iii) cut withBamHI for cloning intoBamHI-cut λZAP-Express or λDASHII vectors. Partial nucleotide sequencing of the M13 clones followed by detection of open reading frames and their translation allowed the identification of homologues through FASTA searches of the database. Relevant M13 clones were used as probes to isolate corresponding λ phage clones, which could tentatively be mapped to the genome on the basis of presumed genetic collinearity between HHV-7 and HHV-6. Genomic ‘walking’ betweenEcoRI andBamHI λ genomic libraries enabled overlapping neighbouring clones to be identified and mapped. Each of these clones was analysed to mapBamHI,EcoRI,Sall,Smal andXhol restriction endonuclease sites to provide complete endonuclease maps for the entire genome.
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急疹和人类疱疹病毒 7 型感染。
DOI：
10.1097/00006454-199411000-00016
发表时间：
1994
期刊：
The Pediatric infectious disease journal
影响因子：
--
作者：
Y. Hidaka;K. Okada;K. Kusuhara;C. Miyazaki;K. Tokugawa;K. Ueda
通讯作者：
K. Ueda
人 B 淋巴细胞病毒（人疱疹病毒 6）的体外细胞趋向性
影响因子：
15.3
作者：
P. Lusso;P. Markham;E. Tschachler;F. Di;Marzo Veronese;I. S. Zaki Salahuddin;D. Ablashi;S. Pahwa;K. Krohn;R. Gallo
通讯作者：
R. Gallo
鉴定映射到人类疱疹病毒 6 推定的早期基因座的反式激活功能
影响因子：
5.4
作者：
Michelle E. D. Martin;John Nicholas;B. Thomson;C. Newman;R. Honess
通讯作者：
R. Honess
原发性人类疱疹病毒 7 型感染婴儿的临床特征和病毒排泄。
影响因子：
8
作者：
Y. Asano;S. Suga;T. Yoshikawa;T. Yazaki;T. Uchikawa
通讯作者：
T. Uchikawa
人类疱疹病毒 6 中人类巨细胞病毒 US22 基因家族同源物的鉴定。
影响因子：
3.8
作者：
S. Efstathiou;G. Lawrence;Chris M. Brown;B. Barrell
通讯作者：
B. Barrell