Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition
Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition
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斜纹夜蛾 OR18 的鉴定和表达谱，该基因在嗅觉识别中具有潜在作用
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Yongjun Du
中科院分区：
文献类型：
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作者：
Kaidi Zheng;Xinda Lin;Yongjun Du
利用RACE(快速扩增基因末端扩增)和PCR(聚合酶链式反应)的同源克隆策略，获得了斜纹夜蛾Spotoptera litura(Fabricius)(夜蛾科)触角中一个候选嗅觉受体基因SlituOR18的全长cDNA。SlituOR18蛋白与其他夜蛾嗅觉受体18(OR18)蛋白有80%的序列同源性和7个相同的跨膜区。SlituOR18也具有与SlituORco相似的结构，编码斜纹夜蛾中的嗅觉共同受体。跨膜片段IV和V之间的序列比其他跨膜片段之间的序列更长，N末端是胞质内的。QRT-PCR分析表明，SlituOR18主要在成蛾中表达，雌性触角的表达高于雄性触角。SlituOR18在卵中表达较弱，在4龄和5日龄蛹的触角中未见表达。原位杂交实验表明，这些受体类型的表达明显局限于毛型嗅觉感受器(Trichodeum)的碱基，已知这种感受器含有对食物气味或信息素敏感的神经元。我们的数据表明，SlituORco和SlituOR18的相对表达水平都表现出日变化。SlituOR18的表达在利用合成信息素诱饵诱捕的斜纹夜蛾的不同地理种群之间存在差异。来自四川、广西和湖南的斜纹夜蛾种群间SlituOR18的mRNA表达水平相似，但高于上海和宁波的种群。我们认为OR18可能在夜蛾的嗅觉中起关键作用，是未来新的害虫管理策略的潜在靶标。
The full‐length cDNA of the gene SlituOR18, encoding a candidate olfactory receptor in the antennae of the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera Noctuidae), was identified through homology cloning strategies using RACE (rapid amplification of cDNA ends) and PCR (polymerase chain reaction). The protein of SlituOR18 shared >80% sequence identity and the same seven transmembrane domains with other olfactory receptor 18 (OR18) proteins sequenced from noctuid moths. SlituOR18 also had a similar structure to SlituORco, encoding the olfactory co‐receptor in S. litura. The sequence between transmembrane segments IV and V was longer than other sequences between transmembrane segments, and the N‐terminus was intracytoplasmic. Analysis by qRT‐PCR showed SlituOR18 was predominantly expressed in adult moths and there was higher expression in female antennae than in male antennae. Weak expression of SlituOR18 was observed in eggs and no expression was observed in the antennae of fourth instars and 5‐day‐old pupae. In situ hybridization experiments revealed that expression of these receptor types was clearly restricted to the bases of trichodea‐type olfactory sensilla (sensillum trichodeum), that are known to contain neurons sensitive to food odor or pheromones. Our data demonstrated that both SlituORco and SlituOR18 showed diurnal changes in their relative expression level. Expression of SlituOR18 varied among geographic populations of S. litura that had been trapped in the field using synthetic pheromone lures. The mRNA expression level of SlituOR18 was similar among S. litura populations from Sichuan, Guangxi, and Hunan, and higher than in populations from Shanghai and Ningbo. We suggest that OR18 could play a critical role in olfaction in noctuid moths and is a potential target for novel pest management strategies in the future.
DOI：
10.1073/pnas.1017963108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Grosse-Wilde, Ewald;Kuebler, Linda S.;Hansson, Bill S.
通讯作者：
Hansson, Bill S.