Light regulation of retinal dopamine that is independent of melanopsin phototransduction.
Light regulation of retinal dopamine that is independent of melanopsin phototransduction.
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DOI：
10.1111/j.1460-9568.2009.06631.x
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Lucas RJ
中科院分区：
文献类型：
--
作者：
Cameron MA;Pozdeyev N;Vugler AA;Cooper H;Iuvone PM;Lucas RJ
视网膜中多巴胺的光依赖性释放是光适应机制的重要组成部分。在生理和解剖研究中，含有黑视素的视网膜内光感受器已被证明与DA - amacrine细胞进行物理接触，并参与局部视网膜环境的调节。在这里，我们通过DA与其主要代谢物DOPAC的比例以及酪氨酸羟化酶（TH）标记的DA无分泌细胞中c-fos的诱导来确定它们是否有助于视网膜中DA的光调节。光处理（约0.7 log W/m2，持续90min）导致DA释放大幅增加（DOPAC:DA比值增加），并在野生型小鼠和缺乏黑视素（Opn4-/-）的小鼠中广泛诱导DA无毛细胞中的核c-fos。在缺乏光导杆（Gnat1-/-）的小鼠中，光诱导的DA释放也得到了保留，尽管与野生型相比，这种反应的强度大大降低，在DAergic amacrines中光依赖性核c-fos的发生率也有所降低。相比之下，缺乏视杆细胞和视锥细胞的小鼠的DAergic系统（rd/rd cl）没有可检测到的光响应。我们的数据表明，DA（一种关键的视网膜神经调节剂）的光调节主要来源于视杆细胞和视锥细胞，而黑视素对于这种光反应既不是必要的，也不是充分的。
Light dependent release of dopamine (DA) in the retina is an important component of light-adaptation mechanisms. Melanopsin-containing inner retinal photoreceptors have been shown to make physical contacts with DA amacrine cells and have been implicated in the regulation of the local retinal environment in both physiological and anatomical studies. Here we determined whether they contribute to photic regulation of DA in the retina as assayed by the ratio of DA with its primary metabolite, DOPAC, and by c-fos induction in tyrosine hydroxylase (TH) labeled DA amacrine cells. Light treatment (∼0.7 log W/m2 for 90min) resulted in a substantial increase in DA release (as revealed by an increase in DOPAC:DA ratio) as well as widespread induction of nuclear c-fos in DA amacrine cells in wild type mice and in mice lacking melanopsin (Opn4-/-). Light induced DA release was also retained in mice lacking rod phototransduction (Gnat1-/-), although the magnitude of this response was substantially reduced compared to wild-types, as was the incidence of light-dependent nuclear c-fos in DAergic amacrines. By contrast, the DAergic system of mice lacking both rods and cones (rd/rd cl) showed no detectable light response. Our data suggest that light regulation of DA, a pivotal retinal neuromodulator, originates primarily with rods and cones and that melanopsin is neither necessary nor sufficient for this photoresponse.
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DOI：
10.1073/pnas.250478897
发表时间：
2000-12-05
影响因子：
11.1
作者：
Calvert, PD;Krasnoperova, NV;Lem, J
通讯作者：
Lem, J
影响因子：
6.1
作者：
MORGAN, WW;KAMP, CW
通讯作者：
KAMP, CW
影响因子：
9.2
作者：
Sekaran, S;Foster, RG;Hankins, MW
通讯作者：
Hankins, MW
影响因子：
64.8
作者：
Gueler, Ali D.;Ecker, Jennifer L.;Hattar, Samer
通讯作者：
Hattar, Samer
影响因子：
4.7
作者：
PARKINSON, D;RANDO, RR
通讯作者：
RANDO, RR