Effects of Chinese herbal monomers on oxidative phosphorylation and membrane potential in cerebral mitochondria isolated from hypoxia-exposed rats in vitro.
Effects of Chinese herbal monomers on oxidative phosphorylation and membrane potential in cerebral mitochondria isolated from hypoxia-exposed rats in vitro.
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中药单体对离体缺氧大鼠脑线粒体氧化磷酸化和膜电位的影响
DOI：
10.3969/j.issn.1673-5374.2012.27.003
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发表时间：
2012-09-25
影响因子：
6.1
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Yan W;Liu J
线粒体功能障碍是高原缺氧性脑损伤的重要发病机制。某些中药单体可能通过提高氧化磷酸化效率发挥抗缺氧作用。本研究通过观察10种中药单体对缺氧大鼠脑线粒体呼吸和膜电位的影响，筛选抗缺氧药物。将大鼠暴露于405.35 mm Hg（54.04 kPa）低压环境中3 d，建立高原低氧模型。分离脑线粒体，分别用不同浓度的中药单体（青藤碱、水飞蓟素、甘草酸、黄芩苷、槲皮素、银杏内酯B、苦参碱、胡椒碱、甘草皂苷Rg 1和氧化苦参碱）处理5 min。分别用Clark氧电极和罗丹明123荧光分析法测定线粒体耗氧量和膜电位。低氧暴露显著降低状态3呼吸频率、呼吸控制率和线粒体膜电位，显著升高状态4呼吸频率。在体外培养的缺氧大鼠脑线粒体中，丹参酮、丹参Rg1和氧化苦参碱以剂量依赖的方式增加线粒体的呼吸控制率，而丹参Rg1、胡椒碱和氧化苦参碱则显著增加缺氧大鼠脑线粒体的膜电位。因此，中药单体甘草酸、甘草皂苷Rg1、胡椒碱和氧化苦参碱可改善体外低压缺氧诱导的脑线粒体氧化磷酸化障碍。
Mitochondrial dysfunction is the key pathogenic mechanism of cerebral injury induced by high-altitude hypoxia. Some Chinese herbal monomers may exert anti-hypoxic effects through enhancing the efficiency of oxidative phosphorylation. In this study, effects of 10 kinds of Chinese herbal monomers on mitochondrial respiration and membrane potential of cerebral mitochondria isolated from hypoxia-exposed rats in vitro were investigated to screen anti-hypoxic drugs. Rats were exposed to a low-pressure environment of 405.35 mm Hg (54.04 kPa) for 3 days to establish high-altitude hypoxic models. Cerebral mitochondria were isolated and treated with different concentrations of Chinese herbal monomers (sinomenine, silymarin, glycyrrhizic acid, baicalin, quercetin, ginkgolide B, saffron, piperine, ginsenoside Rg1 and oxymatrine) for 5 minutes in vitro. Mitochondrial oxygen consumption and membrane potential were measured using a Clark oxygen electrode and the rhodamine 123 fluorescence analysis method, respectively. Hypoxic exposure significantly decreased the state 3 respiratory rate, respiratory control rate and mitochondrial membrane potential, and significantly increased the state 4 respiratory rate. Treatment with saffron, ginsenoside Rg1 and oxymatrine increased the respiratory control rate in cerebral mitochondria isolated from hypoxia-exposed rats in dose-dependent manners in vitro, while ginsenoside Rg1, piperine and oxymatrine significantly increased the mitochondrial membrane potential in cerebral mitochondria from hypoxia-exposed rats. The Chinese herbal monomers saffron, ginsenoside Rg1, piperine and oxymatrine could thus improve cerebral mitochondrial disorders in oxidative phosphorylation induced by hypobaric hypoxia exposure in vitro.
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影响因子：
4.4
作者：
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通讯作者：
de Agostini, Ariane
影响因子：
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作者：
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通讯作者：
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