Protective effect of puerarin form the roots of Pueraria lobata against systemic inflammatory response syndrome by regulating the levels of related cytokines
Protective effect of puerarin form the roots of Pueraria lobata against systemic inflammatory response syndrome by regulating the levels of related cytokines
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葛根素通过调节相关细胞因子水平对全身炎症反应综合征的保护作用
DOI：
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发表时间：
2011-07
影响因子：
--
通讯作者：
Yan, Jie
中科院分区：
文献类型：
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作者：
Quan, Sheng;Wang, Jun-bo;Xia, Xiao-ping;Lin, Xv-ai;Yan, Jie
The outcome of systemic inflammatory response syndrome (SIRS) is poor due to unclear pathogenesis and unsatisfied therapeutic strategies. Therefore, to understand the pathogenetic mechanism and screen novel drugs are critical for the improvement of the therapeutic efficacy of SIRS. In the present study, to determine the protective mechanism of puerarin on SIRS, Sprague Dawley (SD) rats were intraperitoneally injected with different doses of zymosan-A to generate an experimental SIRS animal model. The peripheral TNF-α, IL-6 and IL-10 levels of SIRS rats were measured using quantitative ELISA assay. Protective effects puerarin on SIRS rats via regulating cytokine levels was subsequently determined. The results showed about 75.0% of SIRS rats died after injection with 1000 mg/kg zymosan-A, whereas only 16.7% of SIRS rats (1000 mg/kg zymosan-A) were found dead after the treatment with 62.5 mg/kg puerarin (P< 0.01 vs SIRS group). 24 h after injection with 750 mg/kg zymosan-A, the peripheral levels of TNF-α, IL-6 and IL-10 were 30.87±6.81 pg/ml, 525.20±92.45 pg/ml and 1.37±0.17 ng/ml, respectively. However, the levels of TNF-α and IL-6 (16.71±3.75 pg/ml and 399.30±77.87 pg/ml, respectively) were significantly lowered (P< 0.01) and IL-10 level (1.95±0.17 ng/ml) was markedly elevated (P< 0.01) after treatment with 62.5 mg/kg puerarin when compared with those in SIRS group. Puerarin conferred protective effects on the SIRS via down-regulating pro-inflammatory TNF-α and IL-6 as well as up-regulating anti-inflammatory IL-10.
Key words: Experimental SIRS, puerarin, protective effects, cytokines.
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DOI：
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发表时间：
2007-08
期刊：
Biomedical and environmental sciences : BES
影响因子：
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作者：
Xiaohong Xu;Xiao-xiang Zheng;Qiong Zhou;Hui Li
通讯作者：
Xiaohong Xu;Xiao-xiang Zheng;Qiong Zhou;Hui Li
影响因子：
2.2
作者：
Ferrer, TJ;Webb, JW;Cone, JB
通讯作者：
Cone, JB
影响因子：
3.1
作者：
A. Baue
通讯作者：
A. Baue
DOI：
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发表时间：
1996-03
期刊：
Zhongguo yao li xue bao = Acta pharmacologica Sinica
影响因子：
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作者：
H. Ji;T. Y. Wang
通讯作者：
H. Ji;T. Y. Wang
DOI：
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发表时间：
1995-09
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
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作者：
L. Chen;Q. Chai;A. Zhao;X. Chai
通讯作者：
L. Chen;Q. Chai;A. Zhao;X. Chai