HIGH CROSSOVER RATE1 encodes PROTEIN PHOSPHATASE X1 and restricts meiotic crossovers in Arabidopsis.
HIGH CROSSOVER RATE1 encodes PROTEIN PHOSPHATASE X1 and restricts meiotic crossovers in Arabidopsis.
复制标题
DOI：
10.1038/s41477-021-00889-y
复制
发表时间：
2021-04
期刊：
影响因子：
18
通讯作者：
Henderson IR
中科院分区：
文献类型：
--
作者：
Nageswaran DC;Kim J;Lambing C;Kim J;Park J;Kim EJ;Cho HS;Kim H;Byun D;Park YM;Kuo P;Lee S;Tock AJ;Zhao X;Hwang I;Choi K;Henderson IR
尽管存在过量的起始DNA双链断裂，减数分裂交换在大多数真核生物中受到严格限制。大多数植物交换依赖于 I 类干扰修复，少数通过 II 类途径形成。 II 类修复受到抗重组途径的限制，但抑制 I 类交叉的类似途径尚不清楚。我们使用荧光交叉报告基因在拟南芥中进行了正向遗传筛选，以识别重组频率增加或减少的突变体。我们将高交叉率 1 (HCR1) 确定为抑制交叉并编码蛋白质磷酸酶 X1。全基因组分析表明，远端染色体臂中的 hcr1 交叉增加。 hcr1 中的 MLH1 病灶显着增加，交叉干扰减少，表明对 I 类修复有影响。酵母双杂交和植物内检测一致显示 HCR1 和 I 类蛋白（包括 HEI10、PTD、MSH5 和 MLH1）之间存在相互作用。我们认为 HCR1 在对抗促重组激酶以限制拟南芥中的交叉方面发挥着重要作用。
Meiotic crossovers are tightly restricted in most eukaryotes, despite an excess of initiating DNA double-strand breaks. The majority of plant crossovers are dependent on Class I interfering repair, with a minority formed via the Class II pathway. Class II repair is limited by anti-recombination pathways, however similar pathways repressing Class I crossovers are unknown. We performed a forward genetic screen in Arabidopsis using fluorescent crossover reporters, to identify mutants with increased or decreased recombination frequency. We identified HIGH CROSSOVER RATE1 (HCR1) as repressing crossovers and encoding PROTEIN PHOSPHATASE X1. Genome-wide analysis showed that hcr1 crossovers are increased in the distal chromosome arms. MLH1 foci significantly increase in hcr1 and crossover interference decreases, demonstrating an effect on Class I repair. Consistently, yeast two-hybrid and in planta assays show interaction between HCR1 and Class I proteins, including HEI10, PTD, MSH5, and MLH1. We propose that HCR1 plays a major role in opposition to pro-recombination kinases to restrict crossovers in Arabidopsis.
登录
查看更多内容
影响因子：
64.8
作者：
Garcia V;Gray S;Allison RM;Cooper TJ;Neale MJ
通讯作者：
Neale MJ
影响因子：
16
作者：
Chowdhury, Dipanjan;Xu, Xingzhi;Zhong, Xueyan;Ahmed, Fariyal;Zhong, Jianing;Liao, Ji;Dykxhoorn, Derek M.;Weinstock, David M.;Pfeifer, Gerd P.;Lieberman, Judy
通讯作者：
Lieberman, Judy
影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
影响因子：
4.5
作者：
Choi K;Reinhard C;Serra H;Ziolkowski PA;Underwood CJ;Zhao X;Hardcastle TJ;Yelina NE;Griffin C;Jackson M;Mézard C;McVean G;Copenhaver GP;Henderson IR
通讯作者：
Henderson IR
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM