Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis.
Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis.
复制标题
DOI：
10.3892/or.2012.1634
复制
发表时间：
2012-04
期刊：
影响因子：
4.2
通讯作者：
Zhang XH
中科院分区：
文献类型：
--
作者：
Zheng SR;Guo GL;Zhang W;Huang GL;Hu XQ;Zhu J;Huang QD;You J;Zhang XH
越来越多的证据表明，miRNAs在肿瘤发生中起着至关重要的作用。miR-155是最多功能的mirna之一，其过表达已被发现与包括乳腺癌在内的不同类型的癌症相关。为了进一步确定miR-155在乳腺癌中的潜在作用，我们通过real-time PCR评估了miR-155的表达水平，并将结果与临床病理特征相关联。采用real-time PCR分析42例浸润性导管癌和3例浸润性小叶癌匹配的非肿瘤组织和肿瘤组织中miR-155的表达情况。此外，我们使用反义技术在体外抑制miR-155的表达。采用WST-8试验评估细胞活力，采用凋亡试验研究miR-155反义寡核苷酸（miR-155 ASO）对HS578T细胞死亡的影响。miR-155在肿瘤组织中的表达水平明显高于配对的非肿瘤组织（P<0.001）。miR-155表达上调与淋巴结阳性（P=0.034）、高增殖指数（Ki-67 >10%） （P=0.019）和晚期乳腺癌TNM临床分期（P=0.002）相关。有趣的是，我们接下来发现miR-155的表达水平与ER状态（P=0.041）和PR状态（P=0.029）密切相关。流式细胞术检测转染效率高于70%，WST-8检测显示，转染miR-155 ASO后，与scramble （SCR）组或脂质体组相比，HS578T细胞的活力大大降低。Annexin V-FITC/PI检测也表明转染miR-155 ASO促进细胞凋亡。
Accumulating evidence shows that mircroRNAs (miRNAs) play a vital role in tumorigenesis. miR-155 is one of the most multifunctional miRNAs whose overexpression has been found to be associated with different types of cancer including breast cancer. To further determine the potential involvement of miR-155 in breast cancer, we evaluated the expression levels of miR-155 by real-time PCR and correlated the results with clinicopathological features. Matched non-tumor and tumor tissues of 42 infiltrating ductal carcinomas and 3 infiltrating lobular carcinomas were analyzed for miR-155 expression by real-time PCR. Further, we used an antisense technique to inhibit miR-155 expression in vitro. WST-8 test was performed to evaluate cell viability and apoptosis assay was used to investigate the effect of the miR-155 antisense oligonucleotide (miR-155 ASO) on HS578T cell death. The expression levels of miR-155 were significantly higher in tumor tissues than the levels in matched non-tumor tissues (P<0.001). Up-regulated miR-155 expression was associated with lymph node positivity (P=0.034), higher proliferation index (Ki-67 >10%) (P=0.019) and advanced breast cancer TNM clinical stage (P=0.002). Interestingly, we next found that miR-155 expression levels had close relations with ER status (P=0.041) and PR status (P=0.029). Transfection efficiency detected by flow cytometry was higher than 70%, the WST-8 test showed that viability of HS578T cells was greatly reduced after transfection with miR-155 ASO compared with the scramble (SCR) group or the liposome group. The Annexin V-FITC/PI assay also indicated that transfection with miR-155 ASO promoted apoptosis.
登录
查看更多内容
影响因子：
14.9
作者：
Lu J;Tsourkas A
通讯作者：
Tsourkas A
影响因子：
32.4
作者：
O'Connell RM;Kahn D;Gibson WS;Round JL;Scholz RL;Chaudhuri AA;Kahn ME;Rao DS;Baltimore D
通讯作者：
Baltimore D
影响因子：
2.1
作者：
Juan, David;Alexe, Gabriela;Liou, Louis S.
通讯作者：
Liou, Louis S.
影响因子：
11.1
作者：
Pedersen, Irene M.;Otero, Dennis;Kao, Elaine;Miletic, Ana V.;Hother, Christoffer;Ralfkiaer, Elisabeth;Rickert, Robert C.;Gronbaek, Kirsten;David, Michael
通讯作者：
David, Michael
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ