Regulation of myo-inositol catabolism by a GntR-type repressor SCO6974 in Streptomyces coelicolor
Regulation of myo-inositol catabolism by a GntR-type repressor SCO6974 in Streptomyces coelicolor
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天蓝色链霉菌中 GntR 型阻遏物 SCO6974 对肌醇分解代谢的调节
DOI：
10.1007/s00253-014-6368-1
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Gang Liu
中科院分区：
文献类型：
--
作者：
Wenyan Gao;Yuanyuan Pan;Huarong Tan;Gang Liu
肌醇对链霉菌的生长和形态分化有重要作用。基因组序列分析显示天蓝色链霉菌中存在一个肌醇分解代谢基因簇。破坏该簇中的相应基因，就可以消除细菌在肌醇作为单一碳源上的生长。在基本培养基中添加肌醇可显著增强这些基因的转录。一个推定的调控基因SCO 6974，编码GntR家族蛋白，位于该簇中。SCO 6974的破坏显著增强了肌醇分解代谢基因的转录。SCO 6974被证明与肌醇分解代谢基因的启动子区域使用电泳迁移率变动分析。DNA酶I足迹分析表明SCO 6974识别一个保守的回文序列（A/T）TGT（A/C）N（G/T）（G/T）ACA（A/T）。保守序列的碱基替换完全消除了SCO 6974与靶标的结合，表明SCO 6974直接抑制肌醇分解代谢基因的转录。此外，SCO 6974的破坏与S.在甘露醇-大豆粉培养基中培养腔肠藻，并过量产生放线菌紫素和钙依赖性抗生素。另外，肌醇抑制了突变体的孢子形成缺陷，表明该效果可能与肌醇的短缺有关，由于其在该菌株中增强的catenosylation。这种增强的肌醇催化剂可能产生二羟丙酮磷酸和乙酰辅酶A，它们是过度产生的抗生素的间接或直接前体。
Myo-inositol is important for Streptomyces growth and morphological differentiation. Genomic sequence analysis revealed a myo-inositol catabolic gene cluster in Streptomyces coelicolor. Disruption of the corresponding genes in this cluster abolished the bacterial growth on myo-inositol as a single carbon source. The transcriptions of these genes were remarkably enhanced by addition of myo-inositol in minimal medium. A putative regulatory gene SCO6974, encoding a GntR family protein, is situated in the cluster. Disruption of SCO6974 significantly enhanced the transcription of myo-inositol catabolic genes. SCO6974 was shown to interact with the promoter regions of myo-inositol catabolic genes using electrophoretic mobility shift assays. DNase I footprinting assays demonstrated that SCO6974 recognized a conserved palindromic sequence (A/T)TGT(A/C)N(G/T)(G/T)ACA(A/T). Base substitution of the conserved sequence completely abolished the binding of SCO6974 to the targets demonstrating that SCO6974 directly represses the transcriptions of myo-inositol catabolic genes. Furthermore, the disruption of SCO6974 was correlated with a reduced sporulation of S. coelicolor in mannitol soya flour medium and with the overproduction of actinorhodin and calcium-dependent antibiotic. The addition of myo-inositol suppressed the sporulation deficiency of the mutant, indicating that the effect could be related to a shortage in myo-inositol due to its enhanced catabolism in this strain. This enhanced myo-inositol catabolism likely yields dihydroxyacetone phosphate and acetyl-CoA that are indirect or direct precursors of the overproduced antibiotics.
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影响因子：
4.4
作者：
M. J. Yebra;M. Zúñiga;Sophie Beaufils;G. Pérez-Martínez;J. Deutscher;V. Monedero
通讯作者：
M. J. Yebra;M. Zúñiga;Sophie Beaufils;G. Pérez-Martínez;J. Deutscher;V. Monedero
链霉菌生长和分化过程中肌醇生物合成的重要性和调控
DOI：
10.1111/j.1365-2958.2012.08000.x
发表时间：
2012-03
期刊：
Mol Microbiol.
影响因子：
--
作者：
Zhang Guohua;Tian Yuqing;Hu Kun;Feng Chi;Keith Chater;Liu Gang;Tan Huarong
通讯作者：
Tan Huarong
DOI：
--
发表时间：
2006-04
期刊：
Journal of biomolecular techniques : JBT
影响因子：
--
作者：
M. Zianni;K. Tessanne;M. Merighi;R. Laguna;F. Tabita
通讯作者：
M. Zianni;K. Tessanne;M. Merighi;R. Laguna;F. Tabita
影响因子：
11.3
作者：
McCormick JR;Flärdh K
通讯作者：
Flärdh K
DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
T. Kieser
通讯作者：
T. Kieser