Estrogen promotes the onset and development of idiopathic scoliosis via disproportionate endochondral ossification of the anterior and posterior column in a bipedal rat model.
Estrogen promotes the onset and development of idiopathic scoliosis via disproportionate endochondral ossification of the anterior and posterior column in a bipedal rat model.
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雌激素通过双足大鼠模型中前后柱不成比例的软骨内骨化促进特发性脊柱侧凸的发生和发展
DOI：
10.1038/s12276-018-0161-7
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发表时间：
2018-11-07
影响因子：
12.8
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Zheng S;Zhou H;Gao B;Li Y;Liao Z;Zhou T;Lian C;Wu Z;Su D;Wang T;Su P;Xu C
本研究旨在通过构建青春期双足大鼠模型，验证雌激素对青春期特发性脊柱侧凸发生发展的影响及其相关机制。进行了实验来研究雷普妥林治疗是否可以预防脊柱侧凸的进展。120只双足大鼠分为雌性组、卵巢切除组、OVX + E2组、雷普妥林组、假组和雄性组。根据脊柱放射学分析，女性和OVX + E2组的脊柱侧凸率和弯曲严重程度高于OVX、Triptorelin和男性组。胸椎CT矢状面测量结果证实，与OVX和Triptorelin组相比，女性组和OVX + E2组T11和T13之间的前节段生长相对较慢，后节段生长较快。组织形态学和免疫组化显示，与雌性和OVX + E2组相比，OVX和Triptorelin组椎软骨生长板增厚带明显变长，表达的X型胶原较多，II型胶原较少。Ki67免疫染色证实，与雌性和OVX + E2组相比，OVX组椎体生长板软骨细胞增殖增加。综上所述，雌激素明显增加了短毛期双足大鼠脊柱侧弯的发生率和弯曲程度。潜在的机制可能是前柱和后柱之间软骨内成骨耦合的丧失。雷公藤雷素降低了双足雌性大鼠脊柱侧凸的发生率和弯曲幅度。
This study aimed to verify the effects of estrogen on the onset and development of adolescent idiopathic scoliosis and the mechanisms associated with these effects by constructing a pubescent bipedal rat model. Experiments were conducted to investigate whether scoliosis progression was prevented by a Triptorelin treatment. One hundred twenty bipedal rats were divided into female, OVX (ovariectomy), OVX + E2, Triptorelin, sham, and male groups. According to a spinal radiographic analysis, the scoliosis rates and curve severity of the female and OVX + E2 groups were higher than those in the OVX, Triptorelin, and male groups. The measurements obtained from the sagittal plane of thoracic vertebrae CT confirmed a relatively slower growth of the anterior elements and a faster growth of the posterior elements between T11 and T13 in the female and OVX + E2 groups than in the OVX and Triptorelin groups. Histomorphometry and immunohistochemistry revealed a significantly longer hypertrophic zone of the vertebral cartilage growth plates that expressed more type X collagen and less type II collagen in the OVX and Triptorelin groups than in the female and OVX + E2 groups. Ki67 immunostaining confirmed an increase in the proliferation of vertebral growth plate chondrocytes in the OVX group compared with the female and OVX + E2 groups. In conclusion, estrogen obviously increased the incidence of scoliosis and curve severity in pubescent bipedal rats. The underlying mechanism may be a loss of coupling of the endochondral ossification between the anterior and posterior columns. Triptorelin decreased the incidence of scoliosis and curve magnitudes in bipedal female rats.
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影响因子：
6.2
作者：
Borjesson, Anna E.;Lagerquist, Marie K.;Ohlsson, Claes
通讯作者：
Ohlsson, Claes
影响因子：
2.7
作者：
Kulis, Aleksandra;Gozdzialska, Anna;Zarzycki, Daniel
通讯作者：
Zarzycki, Daniel
DOI：
10.2106/00004623-199408000-00009
发表时间：
1994-08-01
影响因子：
5.3
作者：
KINDSFATER, K;LOWE, T;AKMAKJIAN, J
通讯作者：
AKMAKJIAN, J
影响因子：
4.1
作者：
Boudreau, M;Courtenay, SC;van der Kraak, GJ
通讯作者：
van der Kraak, GJ
影响因子：
2.8
作者：
Demirkiran, Gokhan;Dede, Ozgur;Acaroglu, Emre
通讯作者：
Acaroglu, Emre