Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis.
Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis.
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DOI：
10.3390/ijms22147749
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发表时间：
2021-07-20
影响因子：
5.6
通讯作者：
Rahman A
中科院分区：
文献类型：
--
作者：
Parveen S;Rahman A
高温胁迫是植物最具威胁性的非生物胁迫之一，在世界范围内限制了作物的生产力。研究表明，植物对中高温的发育反应与生长素的合成和运输调节细胞内生长素的稳态有关。生长素载体蛋白的运输在维持细胞生长素稳态中起着重要作用。细胞内运输很大程度上依赖于细胞骨架成分肌动蛋白，它为囊泡运动提供轨道。不同种类的肌动蛋白及其同工变体在调节植物发育的各个阶段发挥作用。虽然高温改变了细胞内的运输，肌动蛋白在这一过程中的作用仍然不清楚。在这里，我们证明了肌动蛋白7（ACT 7）的同功变异体在调节拟南芥根的中高温反应中起着重要的作用。在中高温条件下，ACT 7的缺失对根伸长的抑制作用增强，而ACT 8的缺失对根伸长的抑制作用不明显。同时，运动学分析表明，高温下act 7 -4突变体的细胞生长速率和细胞伸长显著降低。肌动蛋白动力学的定量分析表明，长时间的中高温调节act 7 -4突变体中丝状肌动蛋白的成束沿着方向和平行性。act 7 -4突变体的过敏反应被发现与细胞内生长素分布的改变有关，这是由于PIN形成的PIN 1和PIN 2外排载体的丰度减少所致。总的来说，这些结果表明，植物类肌动蛋白同功变异体，ACT 7调制长期中高温反应拟南芥根。
High temperature stress is one of the most threatening abiotic stresses for plants limiting the crop productivity world-wide. Altered developmental responses of plants to moderate-high temperature has been shown to be linked to the intracellular auxin homeostasis regulated by both auxin biosynthesis and transport. Trafficking of the auxin carrier proteins plays a major role in maintaining the cellular auxin homeostasis. The intracellular trafficking largely relies on the cytoskeletal component, actin, which provides track for vesicle movement. Different classes of actin and the isovariants function in regulating various stages of plant development. Although high temperature alters the intracellular trafficking, the role of actin in this process remains obscure. Using isovariant specific vegetative class actin mutants, here we demonstrate that ACTIN 7 (ACT7) isovariant plays an important role in regulating the moderate-high temperature response in Arabidopsis root. Loss of ACT7, but not ACT8 resulted in increased inhibition of root elongation under prolonged moderate-high temperature. Consistently, kinematic analysis revealed a drastic reduction in cell production rate and cell elongation in act7-4 mutant under high temperature. Quantification of actin dynamicity reveals that prolonged moderate-high temperature modulates bundling along with orientation and parallelness of filamentous actin in act7-4 mutant. The hypersensitive response of act7-4 mutant was found to be linked to the altered intracellular auxin distribution, resulted from the reduced abundance of PIN-FORMED PIN1 and PIN2 efflux carriers. Collectively, these results suggest that vegetative class actin isovariant, ACT7 modulates the long-term moderate-high temperature response in Arabidopsis root.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
11.6
作者：
Lim, Soohwan;Park, Jeongmoo;Choi, Giltsu
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Choi, Giltsu
影响因子：
9.9
作者：
Kleine-Vehn, Juergen;Wabnik, Krzysztof;Martiniere, Alexandre;Langowski, Lukasz;Willig, Katrin;Naramoto, Satoshi;Leitner, Johannes;Tanaka, Hirokazu;Jakobs, Stefan;Robert, Stephanie;Luschnig, Christian;Govaerts, Willy;Hell, Stefan W.;Runions, John;Friml, Jiri
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Friml, Jiri
影响因子：
11.4
作者：
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通讯作者：
Yu, Hao
DOI：
10.1073/pnas.0808073105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Kleine-Vehn, Juergen;Leitner, Johannes;Friml, Jiri
通讯作者：
Friml, Jiri