Extracellular Vesicle Surface Signatures in IPF Patients: A Multiplex Bead-Based Flow Cytometry Approach.
Extracellular Vesicle Surface Signatures in IPF Patients: A Multiplex Bead-Based Flow Cytometry Approach.
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IPF患者的细胞外囊泡表面特征：一种基于多重微珠的流式细胞术方法。
DOI：
10.3390/cells10051045
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发表时间：
2021-04-28
期刊：
影响因子：
6
通讯作者：
Bargagli E
中科院分区：
文献类型：
--
作者：
d'Alessandro M;Soccio P;Bergantini L;Cameli P;Scioscia G;Foschino Barbaro MP;Lacedonia D;Bargagli E
背景：细胞外囊泡（EVs）是由细胞从其膜分泌的，存在于循环和体液中。EV参与肺部疾病发病机制的知识正在增加。本研究的目的是评估在两个意大利间质性肺疾病转诊中心随访的特发性肺纤维化（IPF）患者队列中外泌体表面表位的表达，并将其与一组健康志愿者进行比较。材料和方法：回顾性选择90例IPF患者（中位年龄和四分位距（IQR）71（66-75）岁; 69例男性）。在开始抗纤维化治疗之前从患者获得血液样品。使用人MACSPlex外泌体试剂盒（Miltenyi Biotec，Bergisch-Gladbach，德国）检测37个外泌体表面表位。结果如下：IPF患者的CD 19、CD 69、CD 8和CD 86显著高于对照组（分别为p = 0.0023、p = 0.0471、p = 0.0082和p = 0.0143）。IPF受试者的CD 42 a低于对照组（p = 0.0153），而IPF患者的CD 209、Cd 133/1、MCSP和ROR 1高于对照组（分别为p = 0.0007、p = 0.0050、p = 0.0139和p = 0.0335）。IPF患者的Kaplan-Meier生存分析：对于中位值和CD 25的临界值0.48，两个亚组的生存率存在显著差异（p = 0.0243，风险比：0.52（95%CI 0.29-0.92）; CD 8也是如此（临界值1.53，p = 0.0309，风险比：1.39（95%CI 0.75-2.53）。结论：我们的多中心研究首次显示了来自IPF患者的EV上的表面表位的表达，提供了关于IPF患者血清中的通信签名/外泌体谱的有趣数据，以及对疾病发病机制的新见解和预测IPF患者中期生存的有希望的可靠性。
Background: Extracellular vesicles (EVs) are secreted by cells from their membrane within circulation and body fluids. Knowledge of the involvement of EVs in pathogenesis of lung diseases is increasing. The present study aimed to evaluate the expression of exosomal surface epitopes in a cohort of idiopathic pulmonary fibrosis (IPF) patients followed in two Italian Referral Centres for Interstitial Lung Diseases, comparing them with a group of healthy volunteers. Materials and Methods: Ninety IPF patients (median age and interquartile range (IQR) 71 (66–75) years; 69 males) were selected retrospectively. Blood samples were obtained from patients before starting antifibrotic therapy. A MACSPlex Exosome Kit, human, (Miltenyi Biotec, Bergisch-Gladbach, Germany), to detect 37 exosomal surface epitopes, was used. Results: CD19, CD69, CD8, and CD86 were significantly higher in IPF patients than in controls (p = 0.0023, p = 0.0471, p = 0.0082, and p = 0.0143, respectively). CD42a was lower in IPF subjects than in controls (p = 0.0153), while CD209, Cd133/1, MCSP, and ROR1 were higher in IPF patients than in controls (p = 0.0007, p = 0.0050, p = 0.0139, and p = 0.0335, respectively). Kaplan-Meier survival analysis for IPF patients: for median values and a cut-off of 0.48 for CD25, the two subgroups showed a significant difference in survival rate (p = 0.0243, hazard ratio: 0.52 (95%CI 0.29–0.92); the same was true for CD8 (cut-off 1.53, p = 0.0309, hazard ratio: 1.39 (95%CI 0.75–2.53). Conclusion: Our multicenter study showed for the first time the expression of surface epitopes on EVs from IPF patients, providing interesting data on the communication signatures/exosomal profile in serum from IPF patients and new insights into the pathogenesis of the disease and a promising reliability in predicting mid-term survival of IPF patients.
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影响因子：
7.3
作者：
Andreu Z;Yáñez-Mó M
通讯作者：
Yáñez-Mó M
影响因子：
5.6
作者：
Bellamri, Nessrine;Morzadec, Claudie;Vernhet, Laurent
通讯作者：
Vernhet, Laurent
影响因子：
6
作者：
Birjandi, Shirin Z.;Palchevskiy, Vyacheslav;BeLperio, John A.
通讯作者：
BeLperio, John A.
影响因子：
6.1
作者：
d'Alessandro, Miriana;Bergantini, Laura;Bargagi, Elena
通讯作者：
Bargagi, Elena
影响因子：
4.7
作者：
Cameli, Paolo;Bergantini, Laura;Bargagli, Elena
通讯作者：
Bargagli, Elena