Genome-wide identification of the GhARF gene family reveals that GhARF2 and GhARF18 are involved in cotton fibre cell initiation.
Genome-wide identification of the GhARF gene family reveals that GhARF2 and GhARF18 are involved in cotton fibre cell initiation.
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GhARF 基因家族的全基因组鉴定揭示 GhARF2 和 GhARF18 参与棉纤维细胞起始
DOI：
10.1093/jxb/ery219
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发表时间：
2018-08-14
影响因子：
6.9
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Xiao G;He P;Zhao P;Liu H;Zhang L;Pang C;Yu J
生长素可以通过GHARF2和GHARF18蛋白激活几个转录因子，以调节棉花纤维细胞的起始。
生长素信号在调节植物发育中起着至关重要的作用。生长素反应因子（ARF）是生长素信号的关键成分，它通过与生长素反应因子元素（Auxres）结合来调节早期生长素反应基因的表达。但是，该基因家族在棉花中没有全面的特征。在这里，我们在组装的山地丘脑基因组中鉴定了56个GHARF基因。该基因家族分为17个亚家族，其中44个成员分布在21个染色体上。 GHARF6和GHARF11亚家族基因主要在营养组织中表达，而Gharf2和Gharf18亚家族基因在种子纤维细胞启动期间高度表达。 GHARF2-1和GHARF18-1专门在毛状体中表达，类似于棉花纤维细胞的器官，并且在拟南芥中这些基因的过表达增强了毛甲曲构的起始。比较转录组分析与辅助预测相结合，显示了11个转录因子是GHARF2和GHARF18的潜在靶基因。这些基因中的六个在种子纤维细胞开始期间显着表达，并在酵母单杂交测定中被GHARF2-1和GHARF18-1结合。我们的结果表明，GHARF2和GHARF18基因可以通过调节几种转录因子基因的表达来成为棉花种子纤维起始的关键调节因子。这项研究加深了我们对生长素介导的棉花纤维细胞启动的理解，并帮助我们在将来繁殖更好的棉花品种。
Auxin may activate several transcription factors via GhARF2 and GhARF18 proteins to regulate cotton seed fibre cell initiation. Auxin signalling plays an essential role in regulating plant development. Auxin response factors (ARFs), which are critical components of auxin signalling, modulate the expression of early auxin-responsive genes by binding to auxin response factor elements (AuxREs). However, there has been no comprehensive characterization of this gene family in cotton. Here, we identified 56 GhARF genes in the assembled Gossypium hirsutum genome. This gene family was divided into 17 subfamilies, and 44 members of them were distributed across 21 chromosomes. GhARF6 and GhARF11 subfamily genes were predominantly expressed in vegetative tissues, whereas GhARF2 and GhARF18 subfamily genes were highly expressed during seed fibre cell initiation. GhARF2-1 and GhARF18-1 were exclusively expressed in trichomes, organs similar to cotton seed fibre cells, and overexpression of these genes in Arabidopsis enhances trichome initiation. Comparative transcriptome analysis combined with AuxRE prediction revealed 11 transcription factors as potential target genes of GhARF2 and GhARF18. Six of these genes were significantly expressed during seed fibre cell initiation and were bound by GhARF2-1 and GhARF18-1 in yeast one-hybrid assays. Our results suggest that GhARF2 and GhARF18 genes may be key regulators of cotton seed fibre initiation by regulating the expression of several transcription factor genes. This study deepens our understanding of auxin-mediated initiation of cotton seed fibre cells and helps us in breeding better cotton varieties in the future.
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影响因子：
46.9
作者：
Li, Fuguang;Fan, Guangyi;Yu, Shuxun
通讯作者：
Yu, Shuxun
影响因子：
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作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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作者：
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