Hypothermia reduces glymphatic transportation in traumatic edematous brain assessed by intrathecal dynamic contrast-enhanced MRI.
Hypothermia reduces glymphatic transportation in traumatic edematous brain assessed by intrathecal dynamic contrast-enhanced MRI.
复制标题
通过鞘内动态对比增强 MRI 评估低温可减少创伤性水肿脑中的类淋巴运输
DOI：
10.3389/fneur.2022.957055
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发表时间：
2022
影响因子：
3.4
通讯作者：
Lu, Qing
中科院分区：
文献类型：
--
作者：
Bai, Yingnan;Yuan, Mingyuan;Mi, Honglan;Zhang, Fengchen;Liu, Xiangyu;Lu, Chen;Bao, Yinghui;Li, Yuehua;Lu, Qing
关键词：
最近的研究表明，淋巴系统可以清除脑细胞外的溶质，在创伤性脑损伤(TBI)后可广泛受损。尽管在动物研究中，低温被认为是一种通过最大限度地减少水肿的形成来保护受损大脑的方法，但关于低温如何影响脑外伤后的淋巴系统，人们知之甚少。我们使用低分子对比剂注入枕大池后的动态增强磁共振成像(DCE-MRI)来跟踪低温治疗的雄性SD大鼠脑损伤后的淋巴转运，并使用弥散加权成像(DWI)序列来识别脑损伤后的水肿，并进一步区分血管源性和细胞毒性水肿。我们发现，低温可以减轻脑水肿，这一点体现在大多数脑区损伤较小，血管源性水肿较少。然而，与减轻脑水肿相反，低温加剧了脑损伤后淋巴运输效率的降低。这种淋巴引流的恶化存在于全脑，表现为大脑半球的不对称性和区域的异质性，与血管源性水肿相关。此外，我们的数据显示，淋巴转运减少和血管源性水肿与减少血管周围水通道蛋白-4(AQP4)的表达密切相关。抑制淋巴转运可能抵消了低温所致的脑水肿减轻的益处，并为脑损伤提供了另一种指示脑损伤的病理生理因素。因此，这项研究提出了一个新的重点，即低温在治疗严重脑外伤中的潜在作用。
The glymphatic system has recently been shown to clear brain extracellular solutes and can be extensively impaired after traumatic brain injury (TBI). Despite hypothermia being identified as a protective method for the injured brain via minimizing the formation of edema in the animal study, little is known about how hypothermia affects the glymphatic system following TBI. We use dynamic contrast-enhanced MRI (DCE-MRI) following cisterna magna infusion with a low molecular weight contrast agent to track glymphatic transport in male Sprague–Dawley rats following TBI with hypothermia treatment and use diffusion-weighted imaging (DWI) sequence to identify edema after TBI, and further distinguish between vasogenic and cytotoxic edema. We found that hypothermia could attenuate brain edema, as demonstrated by smaller injured lesions and less vasogenic edema in most brain subregions. However, in contrast to reducing cerebral edema, hypothermia exacerbated the reduction of efficiency of glymphatic transportation after TBI. This deterioration of glymphatic drainage was present brain-wide and showed hemispherical asymmetry and regional heterogeneity across the brain, associated with vasogenic edema. Moreover, our data show that glymphatic transport reduction and vasogenic edema are closely related to reducing perivascular aquaporin-4 (AQP4) expression. The suppression of glymphatic transportation might eliminate the benefits of brain edema reduction induced by hypothermia and provide an alternative pathophysiological factor indicating injury to the brain after TBI. Thus, this study poses a novel emphasis on the potential role of hypothermia in managing severe TBI.
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影响因子：
12.7
作者：
JIANG, JY;LYETH, BG;POVLISHOCK, JT
通讯作者：
POVLISHOCK, JT
DOI：
10.37349/ent.2022.00023
发表时间：
2022-01-01
期刊：
Exploration of neuroprotective therapy
影响因子：
--
作者：
Quintin, Stephan;Barpujari, Arnav;Lucke-Wold, Brandon
通讯作者：
Lucke-Wold, Brandon
影响因子：
158.5
作者：
Clifton, GL;Miller, ER;Schwartz, M
通讯作者：
Schwartz, M
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
12.4
作者：
Hadaczek, P;Yamashita, Y;Bankiewicz, K
通讯作者：
Bankiewicz, K