Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation.
Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation.
复制标题
DOI：
10.1038/nsmb.2957
复制
发表时间：
2015-03
影响因子：
16.8
通讯作者：
Kunkel, Thomas A.
中科院分区：
文献类型：
--
作者：
Clausen, Anders R.;Lujan, Scott A.;Burkholder, Adam B.;Orebaugh, Clinton D.;Williams, Jessica S.;Clausen, Maryam F.;Malc, Ewa P.;Mieczkowski, Piotr A.;Fargo, David C.;Smith, Duncan J.;Kunkel, Thomas A.
核糖核苷酸在真核生物复制过程中经常掺入DNA中。在这里，我们绘制这些核糖核苷酸的全基因组分布作为芽殖酵母中复制酶学的标记，使用一种新的5′-DNA末端作图方法，水解末端测序。来自核糖核苷酸切除修复缺陷菌株的DNA的HydEn-Seq揭示了核基因组中核糖核苷酸的复制酶和链特异性模式。这些模式支持DNA聚合酶α和δ在滞后链复制中的作用以及DNA聚合酶ε在前导链复制中的作用。他们确定复制起点，终止区和变异的核糖核苷酸掺入频率在整个基因组中，超过三个数量级。HydEn-Seq还揭示了线粒体复制起点处的链特异性5′-DNA末端，表明环状基因组的单向复制。考虑到在DNA中整合和处理核糖核苷酸的酶的保守性，HydEn-Seq可用于跟踪其他生物体中的复制酶学。
Ribonucleotides are frequently incorporated into DNA during eukaryotic replication. Here we map the genome-wide distribution of these ribonucleotides as markers of replication enzymology in budding yeast, using a new 5′-DNA end-mapping method, Hydrolytic End Sequencing. HydEn-Seq of DNA from ribonucleotide excision repair-deficient strains reveals replicase- and strand-specific patterns of ribonucleotides in the nuclear genome. These patterns support the role of DNA polymerases α and δ in lagging strand replication and of DNA polymerase ε in leading strand replication. They identify replication origins, termination zones and variations in ribonucleotide incorporation frequency across the genome that exceed three orders of magnitude. HydEn-Seq also reveals strand-specific 5′-DNA ends at mitochondrial replication origins, suggesting unidirectional replication of a circular genome. Given the conservation of enzymes that incorporate and process ribonucleotides in DNA, HydEn-Seq can be used to track replication enzymology in other organisms.
登录
查看更多内容
影响因子：
16
作者：
Ghodgaonkar, Medini Manohar;Lazzaro, Federico;Olivera-Pimentel, Maite;Artola-Boran, Mariela;Cejka, Petr;Reijns, Martin A.;Jackson, Andrew P.;Plevani, Paolo;Muzi-Falconi, Marco;Jiricny, Josef
通讯作者：
Jiricny, Josef
影响因子：
7.2
作者：
Johansson, Erik;Dixon, Nicholas
通讯作者：
Dixon, Nicholas
DOI：
10.1084/jem.20120876
发表时间：
2012-07-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hiller B;Achleitner M;Glage S;Naumann R;Behrendt R;Roers A
通讯作者：
Roers A
影响因子：
16
作者：
Cerritelli, SM;Frolova, EG;Crouch, RJ
通讯作者：
Crouch, RJ
影响因子：
16
作者：
Fuste, Javier Miralles;Wanrooij, Sjoerd;Falkenberg, Maria
通讯作者：
Falkenberg, Maria