Endogenous miRNA in the green alga Chlamydomonas regulates gene expression through CDS-targeting.
Endogenous miRNA in the green alga Chlamydomonas regulates gene expression through CDS-targeting.
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DOI：
10.1038/s41477-017-0024-6
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发表时间：
2017-10
期刊：
影响因子：
18
通讯作者：
Baulcombe DC
中科院分区：
文献类型：
--
作者：
Chung BY;Deery MJ;Groen AJ;Howard J;Baulcombe DC
微小RNA（miRNA）是存在于许多真核生物中的21-24个核苷酸的RNA，其作为RNA诱导的沉默复合物的一部分调节基因表达。miRNA的序列同一性提供了特异性以引导沉默效应子Argonaute（AGO）蛋白通过碱基配对过程靶向mRNA。AGO复合物促进该靶mRNA的翻译抑制和/或加速衰变。有压倒性的证据表明，在体内和体外翻译抑制起着重要作用。然而，这三种机制中哪一种在体内更重要一直存在争议，特别是当miRNA对内源性基因的影响不能被报告系统忠实地代表时，至少在后生动物中，观察到的抑制远远超过通常观察到的内源性mRNA。在这里，我们提供了一个全面的全球性分析的进化遥远的单细胞绿色衣藻莱茵衣藻量化的影响，miRNA的蛋白质合成和RNA丰度。我们发现，类似于后生动物稳态系统，衣原体内源性miRNA可以通过mRNA的不稳定和翻译抑制来调节基因表达。然而，与多细胞动物miRNA中靶位点利用主要定位于3 'UTR不同，衣原体中利用的靶位点主要位于编码区内。这些结果表明，进化保守的作用模式的miRNA，但细节的机制分歧植物和后生动物王国。
MicroRNAs (miRNA) are 21-24 nucleotide RNAs present in many eukaryotes that regulate gene expression as part of the RNA-induced silencing complex. The sequence identity of the miRNA provides the specificity to guide the silencing effector Argonaute (AGO) protein to target mRNAs via a base pairing process. The AGO complex either promotes translation repression and/or accelerated decay of this target mRNA. There is overwhelming evidence both in vivo and in vitro that translation repression plays a major role. However, there has been controversy about which of these three mechanisms is more significant in vivo, especially when effects of miRNA on endogenous genes cannot be faithfully represented by reporter systems in which, at least in metazoans, the observed repression vastly exceeds that typically observed for endogenous mRNAs. Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. We show that, similar to metazoan steady-state systems, endogenous miRNAs in Chlamydomonas can regulate gene-expression both by destabilization of the mRNA and by translational repression. However, unlike metazoan miRNA where target site utilization localizes mainly to 3'UTRs, in Chlamydomonas utilized target sites lie predominantly within coding regions. These results demonstrate the evolutionarily conserved mode of action for miRNAs, but details of the mechanism diverge between plant and metazoan kingdoms.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1261/rna.052548.115
发表时间：
2015-10
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Chung BY;Hardcastle TJ;Jones JD;Irigoyen N;Firth AE;Baulcombe DC;Brierley I
通讯作者：
Brierley I
影响因子：
11.4
作者：
Mallory, AC;Reinhart, BJ;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
DOI：
10.1126/science.1215691
发表时间：
2012-04-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Djuranovic S;Nahvi A;Green R
通讯作者：
Green R