Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.

Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.
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DOI:
10.1038/nmeth.1429
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发表时间:
2010-03
期刊:
影响因子:
48
通讯作者:
Kohl, Peter
Kohl, Peter
中科院分区:
生物学1区
文献类型:
--
作者:
Bub, Gil;Tecza, Matthias;Helmes, Michiel;Lee, Peter;Kohl, Peter

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我们介绍了一种成像方式,通过偏移像素曝光时间在捕获一个单一的图像帧,嵌入每个帧内的时间信息。这允许在不增加带宽要求的情况下,以本机检测器帧速率同时采集全分辨率图像,并以降低的分辨率采集高速图像序列。使用宏观和微观的例子,包括成像钙瞬变在心脏细胞中,在250赫兹,使用10赫兹百万像素的相机的方法进行了演示。
We introduce an imaging modality that, by offsetting pixel exposure times during capture of a single image frame, embeds temporal information within each frame. This allows simultaneous acquisition of full-resolution images at native detector frame-rates, and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. The method is demonstrated using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250Hz using a 10Hz megapixel camera.
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