Sequence conservation, phylogenetic relationships, and expression profiles of nondigestive serine proteases and serine protease homologs in Manduca sexta.
Sequence conservation, phylogenetic relationships, and expression profiles of nondigestive serine proteases and serine protease homologs in Manduca sexta.
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DOI：
10.1016/j.ibmb.2014.10.006
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发表时间：
2015-07
影响因子：
3.8
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Cao X;He Y;Hu Y;Zhang X;Wang Y;Zou Z;Chen Y;Blissard GW;Kanost MR;Jiang H
丝氨酸蛋白酶（SP）和丝氨酸蛋白酶同源物（SPH）基因编码一个大家族的蛋白质，参与消化，发育，免疫和其他过程。虽然68消化SP和他们的近亲在一个配套文件中报道，我们已经确定了125个其他SP/SPH在天蛾，并研究了它们的结构，进化和表达。其中52个含有用于分子识别的胱氨酸稳定结构，包括clip、LDLa、Sushi、Wonton、TSP、CUB、Frizzle和SR结构域。有19组基因是从相对较近的基因重复和序列分歧进化而来的。35个SP和7个SPH含有1、2或5个剪切结构域。多序列比对和54个片段结构域的分子建模揭示了这些调控模块的结构多样性。通过与果蝇、冈比亚按蚊和赤拟谷盗的同源基因序列比较，将其分为5个亚家族：A为具有1个或5个第3组剪切结构域的SPH，B为具有1个或2个第2组剪切结构域的SPs，C、D1和D2分别为具有1个第1a、1 B和1 c组剪切结构域的SPs。我们将125个SP相关蛋白在脂肪体、脑、中肠、马氏管、睾丸和卵巢不同发育阶段的表达谱分为6类，表明它们参与了各种生理过程。通过基于RNA-Seq的基因注释和表达谱分析，以及基因组内序列比较，我们已经建立了一个框架的信息，为未来的生化研究nondigestive SPs和SPHs在这个模式物种。
Serine protease (SP) and serine protease homolog (SPH) genes in insects encode a large family of proteins involved in digestion, development, immunity, and other processes. While 68 digestive SPs and their close homologs are reported in a companion paper, we have identified 125 other SPs/SPHs in Manduca sexta and studied their structure, evolution, and expression. Fifty-two of them contain cystine-stabilized structures for molecular recognition, including clip, LDLa, Sushi, Wonton, TSP, CUB, Frizzle, and SR domains. There are nineteen groups of genes evolved from relatively recent gene duplication and sequence divergence. Thirty-five SPs and seven SPHs contain 1, 2 or 5 clip domains. Multiple sequence alignment and molecular modeling of the 54 clip domains have revealed structural diversity of these regulatory modules. Sequence comparison with their homologs in Drosophila melanogaster, Anopheles gambiae and Tribolium castaneum allows us to classify them into five subfamilies: A are SPHs with 1 or 5 group-3 clip domains, B are SPs with 1 or 2 group-2 clip domains, C, D1 and D2 are SPs with a single clip domain in group-1a, 1b and 1c, respectively. We have classified into six categories the 125 expression profiles of SP-related proteins in fat body, brain, midgut, Malpighian tubule, testis, and ovary at different stages, suggesting that they participate in various physiological processes. Through RNA-Seq-based gene annotation and expression profiling, as well as intragenomic sequence comparisons, we have established a framework of information for future biochemical research of nondigestive SPs and SPHs in this model species.
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影响因子：
4.8
作者：
Kellenberger, Christine;Leone, Philippe;Roussel, Alain
通讯作者：
Roussel, Alain
影响因子：
2.9
作者：
Huang, Rudan;Lu, Zhiqiang;Jiang, Haobo
通讯作者：
Jiang, Haobo
影响因子：
2.9
作者：
PALLAGHY, PK;SCANLON, MJ;NORTON, RS
通讯作者：
NORTON, RS
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
影响因子：
3.8
作者：
Jiang, HB;Wang, Y;Kanost, M
通讯作者：
Kanost, M