The juvenile hormone analogue methoprene up-regulates the Ha-RNA-binding protein
The juvenile hormone analogue methoprene up-regulates the Ha-RNA-binding protein
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保幼激素类似物甲氧普林上调 Ha-RNA 结合蛋白
DOI：
10.1016/j.mce.2010.12.029
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发表时间：
2011-02
影响因子：
4.1
通讯作者：
Zhao, Xiao-Fan
中科院分区：
文献类型：
--
作者：
Yang, Xiao-Hui;Liu, Peng-Cheng;Zheng, Wei-Wei;Wang, Jin-Xing;Zhao, Xiao-Fan
RNA结合蛋白（rbp）与细胞内的RNA结合形成核糖核蛋白（RNP）复合物。rbp参与RNA代谢的各个方面，但它们在少年激素（JH）信号通路中的作用尚不清楚。从鳞翅目昆虫棉铃虫（Helicoverpa armigera）中获得了一种rna结合蛋白（Ha-RBP），该蛋白可被幼虫激素类似物甲基戊二烯在mRNA水平上上调。免疫组化结果显示，Ha-RBP主要分布在蛋白合成活跃的细胞中，其敲除降低了jh应答基因的蛋白水平。免疫细胞化学结果显示，Ha-RBP在正常细胞的细胞核和细胞质中均有分布，甲基戊二烯可促进Ha-RBP从细胞核向细胞质的易位。这一过程是由JH受体候选HaMet介导的，而不是由超螺旋蛋白（USP）介导的。RNAi敲除HaMet可降低Ha-RBP的表达，阻断其从细胞核向细胞质的转运。这些发现表明，Ha-RBP参与了幼体激素信号通路，Met通过调节Ha-RBP从细胞核到细胞质的易位介导JH信号通路，这可能允许Ha-RBP修饰蛋白质翻译。
RNA-binding proteins (RBPs) associate with RNA in cells to form ribonucleoprotein (RNP) complexes. The RBPs are involved in various aspects of RNA metabolism, but their roles in the juvenile hormone (JH) signaling pathway are not well known. An RNA-binding protein (Ha-RBP) was obtained from Helicoverpa armigera, a lepidopteran insect, which could be up-regulated by the juvenile hormone analogue methoprene at the mRNA level. Immunohistochemistry showed that Ha-RBP was mainly distributed in cells where protein synthesis was active, and its knockdown decreased the protein levels of JH-responsive genes. Immunocytochemistry showed that Ha-RBP was located in both the nucleus and the cytoplasm of normal cells, and methoprene could promote the translocation of Ha-RBP from the nucleus to the cytoplasm. This process was mediated by the JH receptor candidate HaMet but not by the ultraspiracle protein (USP). The knockdown of HaMet by RNAi decreased the expression of Ha-RBP and blocked its translocation from the nucleus to the cytoplasm. Together these findings indicate that Ha-RBP is involved in the juvenile hormone signaling pathway and Met mediates JH signaling by regulating Ha-RBP translocation from nucleus to cytoplasm, which may allow Ha-RBP to modify protein translation.
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影响因子：
64.8
作者：
Hachet, O;Ephrussi, A
通讯作者：
Ephrussi, A
DOI：
10.1007/bf01957476
发表时间：
1989-04
期刊：
Experientia
影响因子：
--
作者：
V. Sevala;K. Davey
通讯作者：
V. Sevala;K. Davey
影响因子：
10.5
作者：
Blencowe, BJ;Issner, R;Sharp, PA
通讯作者：
Sharp, PA
影响因子：
4.6
作者：
Xiaofeng Zhou;L. Riddiford
通讯作者：
Xiaofeng Zhou;L. Riddiford
影响因子：
11.4
作者：
Le Hir, H;Izaurralde, E;Moore, MJ
通讯作者：
Moore, MJ