Phase stability and biological property evaluation of plasma sprayed hydroxyapatite coatings for orthopedic and dental applications.
Phase stability and biological property evaluation of plasma sprayed hydroxyapatite coatings for orthopedic and dental applications.
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DOI：
10.1016/j.actbio.2015.01.022
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发表时间：
2015-04
影响因子：
9.7
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Vahabzadeh, Sahar;Roy, Mangal;Bandyopadhyay, Amit;Bose, Susmita
关键词：
在这项工作中，我们研究了锶（Sr）掺杂剂对等离子喷涂羟基磷灰石（HA）涂层的体外蛋白质释放动力学和体内成骨性能的影响，沿着它们的溶解行为。等离子喷涂HA涂层广泛应用于承重种植体。除了骨整合外，新一代HA涂层有望递送可诱导骨诱导的生物分子和/或药物。本文报道了结晶和非晶HA涂层的制备商业纯钛（CP-Ti）使用电感耦合射频（RF）等离子喷涂，在不同的溶液pH值和它们的稳定性。涂层制备在110 mm的工作距离从喷嘴显示平均钙离子释放18和90 ppm的中性和酸性环境中，分别。将工作距离减小至90 mm导致形成具有较少结晶HA和具有较高溶度积的相的涂层，因此在32天内溶解度较高。在pH值为7.4的磷酸盐缓冲液中，Sr掺杂的HA涂层测得模型蛋白牛血清白蛋白（BSA）的92%释放，而纯HA涂层只能测得72%释放。BSA在涂层上吸附过程中的变形表明蛋白质与涂层之间存在较强的相互作用，α-螺旋含量增加。与HA涂层和未涂层Ti植入物相比，早在植入后7周就发现Sr-HA植入物上有类骨质形成。植入12周后，HA植入物上形成类骨质新骨;而Sr-HA样品上开始骨矿化。虽然在裸钛表面上没有形成类骨质，但在植入后16周后，HA和Sr-HA涂层上的骨完全矿化。我们的研究结果表明，相稳定性和化学性质对钛种植体上HA涂层的体外和体内反应都有显著影响。
In this work we have investigated the effects of strontium (Sr) dopant on in vitro protein release kinetics and in vivo osteogenic properties of plasma sprayed hydroxyapatite (HA) coatings, along with their dissolution behavior. Plasma sprayed HA coatings are widely used in load-bearing implants. Apart from osseointegration, the new generation of HA coating is expected to deliver biomolecules and/or drugs that can induce osteoinduction. This paper reports the preparation of crystalline and amorphous HA coatings on commercially pure titanium (Cp-Ti) using inductively coupled radio frequency (RF) plasma spray, and their stability at different solution pH. Coatings prepared at 110 mm working distance from the nozzle showed average Ca ion release of 18 and 90 ppm in neutral and acidic environments, respectively. Decreasing the working distance to 90 mm resulted in the formation of a coating with less crystalline HA and phases with higher solubility products, and consequently higher dissolution over 32 days. A 92% release of a model protein bovine serum albumin (BSA) in phosphate buffer with pH of 7.4 was measured for Sr doped-HA coating, while only a 72% release could be measured for pure HA coating. Distortion of BSA during adsorption on coatings revealed strong interaction between the protein and the coating, with an increase in α-helix content. Osteoid formation was found on Sr-HA implants as early as 7 weeks post implantation compared to HA coated and uncoated Ti implants. After 12 weeks post implantation, osteoid new bone was formed on HA implants; whereas, bone mineralization started on Sr-HA samples. While no osteoid was formed on bare Ti surfaces, bone was completely mineralized on HA and Sr-HA coatings after 16 weeks post implantation. Our results show that both phase stability and chemistry can have significant influence towards in vitro and in vivo response of HA coatings on Ti implants.
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DOI：
10.1021/la903617e
发表时间：
2010-04-06
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Dasgupta S;Banerjee SS;Bandyopadhyay A;Bose S
通讯作者：
Bose S
影响因子：
14
作者：
Chen, QZ;Wong, CT;Luk, KDK
通讯作者：
Luk, KDK
影响因子：
4.1
作者：
Tat, Steeve Kwan;Pelletier, Jean-Pierre;Martel-Pelletier, Johanne
通讯作者：
Martel-Pelletier, Johanne
影响因子：
15.9
作者：
TETI, A;BLAIR, HC;HRUSKA, KA
通讯作者：
HRUSKA, KA
影响因子：
14
作者：
Wang, J;Layrolle, P;de Groot, K
通讯作者：
de Groot, K