Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein.
Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein.
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DOI：
10.1021/la903617e
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发表时间：
2010-04-06
期刊：
影响因子：
--
通讯作者：
Bose S
中科院分区：
文献类型：
--
作者：
Dasgupta S;Banerjee SS;Bandyopadhyay A;Bose S
采用原位沉淀法制备了牛血清白蛋白（BSA）/羟基磷灰石（HA）纳米粒子。以2mol%Zn ~（2+）和Mg ~（2+）为掺杂剂，采用原位合成法合成了Zn ~（2+）/Mg ~（2+）掺杂的HA-BSA纳米粒。在我们的研究中，我们使用BSA作为模型蛋白。研究了掺杂和未掺杂的HA NPs对BSA的摄取量以及随后BSA从NPs中的释放。Zn掺杂的HA NPs显示出最高的BSA摄取量，而未掺杂的HA NPs中负载的BSA量最低。在pH 7.2 ± 0.2的磷酸盐缓冲溶液（PBS）中观察到来自掺杂和未掺杂HA NP的两阶段BSA释放曲线。初始突释是由于BSA从HA表面解吸。缓释的后期阶段由BSA掺入的HA NPs的溶解控制。发现来自Zn掺杂的HA NPs的BSA释放速率最高，而未掺杂的HA NPs以最慢的速率释放BSA。我们的研究表明，从HA纳米颗粒的蛋白质释放速率可以通过添加合适的掺杂剂来控制，并且掺杂的基于HA的纳米颗粒系统可以用于骨生长因子和药物释放研究。
Bovine serum albumin (BSA) protein incorporated with hydroxyapatite (HA) nanoparticles (NPs) were synthesized by in situ precipitation process. 2 mol% Zn2+ and Mg2+ were used as dopants to synthesize Zn2+/Mg2+ doped HA-BSA NPs by in situ synthesis route. In our study we used BSA as a model protein. The amount of BSA uptake by doped and undoped HA NPs and subsequent release of BSA from NPs were investigated. Zn doped HA NPs showed the highest amount of BSA uptake, whereas the amount of BSA loaded in undoped HA NPs was the lowest. A two-stage BSA release profile from doped and undoped HA NPs was observed in phosphate buffer solution (PBS) at pH 7.2 ± 0.2. Initial burst release was due to the desorption of BSA from the HA surface. The later stage of slow release was controlled by the dissolution of BSA incorporated HA NPs. BSA release rate from Zn doped HA NPs was found to be the highest, whereas undoped HA NPs released BSA at the slowest rate. Our study showed that the protein release rate from HA NPs can be controlled by the addition of suitable dopants and doped HA based NP systems can be used in bone growth factor and drug release study.
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10.1016/j.mseb.2007.09.006
发表时间：
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影响因子：
3.6
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影响因子：
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