Correlated firing in tufted cells of mouse olfactory bulb.
Correlated firing in tufted cells of mouse olfactory bulb.
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DOI：
10.1016/j.neuroscience.2010.06.033
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发表时间：
2010-09-15
期刊：
影响因子：
3.3
通讯作者：
Lowe, G.
中科院分区：
文献类型：
--
作者：
Ma, J.;Lowe, G.
时间相关的锋电位放电被认为是重要的编码的嗅觉刺激。在嗅球中，已知两类输出神经元（僧帽细胞和外部簇状细胞）中存在相关尖峰。我们研究了第三类主要的球输出神经元，中间簇状细胞，分析其爆发和尖峰时间的相关性，以及它们与二尖瓣细胞的关系。采用膜片钳和荧光示踪技术，我们记录了自发尖峰从簇状或mitral-tufted细胞对可视化树突状突起在小鼠嗅球切片。我们发现尖峰交叉相关图中的峰值表明在快（峰宽1 ms - 50 ms）和慢（峰宽> 50 ms）时间尺度上的相关活动，仅与会聚的肾小球投射成对。耦合出现更紧密的簇绒对，这表明相关的放电模式和较小的平均宽度和滞后的窄峰。一些窄峰分解成2-3个子峰（宽度1-12 ms），表明快速相关的多种模式。缓慢的相关性与爆裂活动，而快速的相关性是独立的缓慢的相关性，发生在爆裂和非爆裂细胞。AMPA受体拮抗剂NBQX（20 μM）不能消除簇状-簇状或二尖瓣-簇状对中的宽峰或窄峰，NBQX的峰高和峰宽变化与自发漂移无显著差异。因此，AMPA受体不是快速和缓慢尖峰相关性所必需的。电耦合中观察到的所有收敛簇绒和mitral-tufted对测试，这表明间隙连接在协同射击的潜在作用。肾小球特异性相关的尖峰提供了一个有用的机制绑定不同的神经元的输出信号处理和传输不同的感觉信息编码的共同嗅觉受体。
Temporally correlated spike discharges are proposed to be important for the coding of olfactory stimuli. In the olfactory bulb, correlated spiking is known in two classes of output neurons, the mitral cells and external tufted cells. We studied a third major class of bulb output neurons, the middle tufted cells, analyzing their bursting and spike timing correlations, and their relation to mitral cells. Using patch-clamp and fluorescent tracing, we recorded spontaneous spiking from tufted-tufted or mitral-tufted cell pairs with visualized dendritic projections in mouse olfactory bulb slices. We found peaks in spike cross-correlograms indicating correlated activity on both fast (peak width 1 ms – 50 ms) and slow (peak width > 50 ms) time scales, only in pairs with convergent glomerular projections. Coupling appeared tighter in tufted-tufted pairs, which showed correlated firing patterns and smaller mean width and lag of narrow peaks. Some narrow peaks resolved into 2–3 sub-peaks (width 1–12 ms), indicating multiple modes of fast correlation. Slow correlations were related to bursting activity, while fast correlations were independent of slow correlations, occurring in both bursting and non-bursting cells. The AMPA receptor antagonist NBQX (20 μM) failed to abolish broad or narrow peaks in either tufted-tufted or mitral-tufted pairs, and changes of peak height and width in NBQX were not significantly different from spontaneous drift. Thus, AMPA-receptors are not required for fast and slow spike correlations. Electrical coupling was observed in all convergent tufted-tufted and mitral-tufted pairs tested, suggesting a potential role for gap junctions in concerted firing. Glomerulus-specific correlation of spiking offers a useful mechanism for binding the output signals of diverse neurons processing and transmitting different sensory information encoded by common olfactory receptors.
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影响因子：
16.2
作者：
Christie, JM;Bark, C;Westbrook, GL
通讯作者：
Westbrook, GL
DOI：
10.1523/jneurosci.2368-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gire DH;Schoppa NE
通讯作者：
Schoppa NE
影响因子：
2.5
作者：
Chen, WR;Shen, GY;Midtgaard, J
通讯作者：
Midtgaard, J
影响因子：
2.5
作者：
EZEH, PI;WELLIS, DP;SCOTT, JW
通讯作者：
SCOTT, JW
影响因子：
3.3
作者：
AroniadouAnderjaska, V;Ennis, M;Shipley, MT
通讯作者：
Shipley, MT