Angiotensin-II increases cytoplasmic calcium, cell number and total DNA for human periodontal ligamental cells in vitro.
Angiotensin-II increases cytoplasmic calcium, cell number and total DNA for human periodontal ligamental cells in vitro.
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血管紧张素-II 在体外可增加人牙周膜细胞的细胞质钙、细胞数量和总 DNA。
DOI：
10.1111/j.1600-0765.1999.tb02245.x
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发表时间：
1999
影响因子：
3.5
通讯作者：
Snowdowne,KW
中科院分区：
文献类型：
--
作者：
Lundergan,WP;Ferry,D;Kobayashi,H;Snowdowne,KW
牙周膜细胞潜在生长因子的鉴定与开发是牙周病原体破坏组织再生的重要新策略。问题是，生长因子很难合成，具有很强的免疫原性，而且价格昂贵。我们的研究目的是确定血管紧张素-II，一种天然存在于心肌中的具有生长因子样特性的八肽，是否也能刺激培养的人牙周韧带细胞。如果血管紧张素-II确实具有生长因子特性，那么它应该能够增加胞浆钙(一种与有丝分裂相关的第二信使)，并促进成纤维细胞的增殖。用Fura-2荧光分光光度法观察细胞内钙离子的变化。细胞计数和DNA总量检测血管紧张素-11诱导的细胞增殖。我们发现血管紧张素-II能增加细胞内钙离子、DNA总量和细胞数，提示血管紧张素-II对人牙周膜细胞具有促有丝分裂作用。
The identification and the development of potential growth factors for the cells of the periodontal ligament is an important and novel stratagem for the regeneration of tissue destroyed by periodontal pathogens. The problem is that growth factors are difficult to synthesize, very immunogenic and expensive. The purpose of our study was to determine if angiotensin‐II, a naturally occurring octo‐peptide with growth‐factor like properties in cardiac muscle, could also stimulate cultured human periodontal ligament cells. If angiotensin‐II does have growth factor properties, then it should be able to increase cytoplasmic calcium (a secondary messenger associated with mitogenesis) and to increase fibroblast proliferation. Fura‐2 fluorescence and aequorin photometry were used to follow changes in cytoplasmic calcium. Cytometry and total amount of DNA were used to measure proliferation evoked by angiotensin‐ll. We found that angiotensin‐II increased cytoplasmic calcium, the total amount of DNA and cell number, suggesting that angiotensin‐II has mitogenic properties for cells isolated from human periodontal ligament.
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影响因子：
4
作者：
TSIEN, RY;RINK, TJ;POENIE, M
通讯作者：
POENIE, M
影响因子：
8.3
作者：
EVERETT, AD;TUFROMCREDDIE, A;GOMEZ, RA
通讯作者：
GOMEZ, RA
影响因子：
5
作者：
W. Schorb;K. Conrad;H. Singer;D. Dostal;K. Baker
通讯作者：
W. Schorb;K. Conrad;H. Singer;D. Dostal;K. Baker
[9] 水母发光蛋白测定细胞内离子钙
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
A. Borle;K. W. Snowdowne
通讯作者：
K. W. Snowdowne
影响因子：
4.3
作者：
MATSUDA, N;LIN, WL;GENCO, RJ
通讯作者：
GENCO, RJ