The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato (Solanum lycopersicum)
The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato (Solanum lycopersicum)
复制标题
非生物胁迫响应 NAC 型转录因子 SlNAC4 调节番茄 (Solanum lycopersicum) 的耐盐性和耐旱性以及胁迫相关基因
DOI：
10.1007/s00299-014-1662-z
复制
发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Z. Hu*
中科院分区：
文献类型：
--
作者：
M. Zhu;G. Chen;J. Zhang;Y. Zhang;Q. Xie;Z. Zhao;Y. Pan;Z. Hu*
关键信息SlNAC4作为一种胁迫响应转录因子，可能有助于提高作物的耐盐性和抗旱性。摘要生物胁迫，特别是盐分和干旱，是显著限制作物生长和生产力的主要因素。植物特异的NAC转录因子在各种逆境反应中发挥重要作用。然而，到目前为止，关于番茄中与胁迫相关的NAC基因的信息很少。在此之前，我们报道了番茄SlNAC4-SlNAC10基因参与了对各种非生物胁迫的响应。表达分析表明，MeJA对SLNAC4也有明显的诱导作用，而ABA对其诱导作用不明显。为了进一步揭示SLNAC4对非生物胁迫的响应功能，我们研究了盐胁迫和干旱胁迫对野生型和转SlNAC4-RNAi番茄植株的影响。结果表明，在萌发后阶段，RNAi植株的根和地上部生长受到盐胁迫的抑制程度高于野生型植株。叶片盐分测定也显示转基因植株的耐性较差，因为与野生型植株相比，转基因植株保持了较低的叶绿素含量。此外，与野生型植株相比，转基因植株对土壤中的盐分和干旱胁迫的耐受性降低，表现为叶片的水分和叶绿素含量较低，失水率较高。值得注意的是，在对照和盐胁迫条件下，SLNAC4-RNAi植株中多种胁迫相关基因的表达均下调。综上所述，这些结果突出了SlNAC4作为一个胁迫响应转录因子的重要作用，它通过一个不依赖于ABA的信号网络，并可能响应生物胁迫，对非生物胁迫的耐性进行正向调节，可能在耐盐和耐旱番茄工程中具有潜在的应用前景。
Key messageSlNAC4 functions as a stress-responsive transcription factor and might be useful for crop salt and drought tolerance improvement.AbstractAbiotic stresses, especially salinity and drought, are major factors that significantly limit crop growth and productivity. Plant-specific NAC transcription factors play crucial roles in various stress responses. However, to date only little information regarding stress-related NAC genes is available in tomato. Previously, we reported that tomatoSlNAC4-SlNAC10genes are involved in response of various abiotic stresses. Expression analysis revealed thatSlNAC4was also induced significantly by MeJA, but not by ABA. To further unravel the function ofSlNAC4in response to abiotic stress, we investigated the effects of salt and drought stress on wild-type andSlNAC4-RNAi transgenic tomato plants. The results demonstrated that the root and shoot growth of RNAi plants was more inhibited by salt stress than that of wild-type at post-germination stage. The leaf salt assay also showed less tolerance in transgenic plants by retaining lower chlorophyll content compared with wild-type plants. In addition, transgenic plants became less tolerant to salt and drought stress in soil, which were demonstrated by lower levels of water and chlorophyll contents, and higher water loss rate in their leaves as compared to wild-type plants under stressed conditions. Notably, the expressions of multiple stress-related genes were downregulated inSlNAC4-RNAi plants under both control and salt-stressed conditions. Collectively, these results highlight the important role of SlNAC4 functions as a stress-responsive transcription factor in positive modulation of abiotic stress tolerance through an ABA-independent signaling networks and possibly in response to biotic stress, and may hold promising applications in the engineering of salt- and drought-tolerant tomato.
登录
查看更多内容
拟南芥 ATAF1 在非生物和生物胁迫反应中的双重功能
影响因子：
44.1
作者：
Deng, Zhiyong;Yang, Cuiping;Wu, Yaorong;Li, Yin;Lai, Jianbin;Zhang, Ling;Yang, Chengwei;Xie, Qi;Zhao, Qingzhen;Zhang, Yiyue;Yin, Bojiao
通讯作者：
Yin, Bojiao
影响因子：
3.1
作者：
L. Tran;Truyen N. Quach;Satish K. Guttikonda;Donavan L. Aldrich;Rajesh Kumar;A. Neelakandan;B. Valliyodan-B.-Va
通讯作者：
L. Tran;Truyen N. Quach;Satish K. Guttikonda;Donavan L. Aldrich;Rajesh Kumar;A. Neelakandan;B. Valliyodan-B.-Va
影响因子：
6.4
作者：
Yimiao Tang;Mei-ying Liu;Shi-Qing Gao;Zhao Zhang;Xin Zhao;Changping Zhao;Feng-ting Zhang;Xue-ping Chen
通讯作者：
Yimiao Tang;Mei-ying Liu;Shi-Qing Gao;Zhao Zhang;Xin Zhao;Changping Zhao;Feng-ting Zhang;Xue-ping Chen
影响因子：
6.2
作者：
Chanjuan Zhang;Junxia Liu;Yuyang Zhang;Xiaofeng Cai;Pengjuan Gong;Junhong Zhang;Taotao Wang;Hanxia Li
通讯作者：
Chanjuan Zhang;Junxia Liu;Yuyang Zhang;Xiaofeng Cai;Pengjuan Gong;Junhong Zhang;Taotao Wang;Hanxia Li
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen