Extragonadal teratocarcinoma derived from embryonal stem cells in chimaeric mice
Extragonadal teratocarcinoma derived from embryonal stem cells in chimaeric mice
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嵌合小鼠胚胎干细胞衍生的性腺外畸胎癌
DOI：
10.1002/path.1711600114
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
M. Hooper
中科院分区：
文献类型：
--
作者：
K. Hardy;P. Carthew;A. Handyside;M. Hooper
研究了将E14TG2a胚胎干（ES）细胞注射到宿主囊胚中所产生的63只嵌合小鼠中的2只（1只雄性和1只雌雄同体）中出现的3种肿瘤。所有的肿瘤都出现在出生后的前3周内。雄性嵌合体的肿瘤和雌雄同体的肿瘤之一位于生殖器周围区域，但位于性腺外。第三个，雌雄同体的小肿瘤在盲肠上。雄性嵌合体的生殖器周围肿瘤是一种畸胎癌，具有多种分化组织，包括非色素视网膜，以及具有高水平碱性磷酸酶活性的未分化胚胎癌（EC）细胞巢。在雌雄同体的生殖器周围肿瘤是一个畸胎瘤，分化较低，没有证据表明EC细胞。葡萄糖磷酸异构酶同工酶分析表明，这两个生殖器周围肿瘤主要是注射的ES细胞，而不是主机芽囊型。这些肿瘤的可能起源，这是第一次报道已经出现在嵌合体小鼠的ES细胞，进行了讨论。
Three tumours which arose in two (one male and one hermaphrodite) out of 63 chimaeric mice resulting from injection of El4TG2a embryo stem (ES) cells into host blastocysts1 have been investigated. All of the tumours appeared within the first 3 weeks after birth. The tumour in the male chimaera and one of the tumours in the hermaphrodite were in the perigenital region but were extragonadal. The third, smaller tumour in the hermaphrodite was on the caecum. The perigenital tumour in the male chimaera was a teratocarcinoma with a wide variety of differentiated tissues, including non‐pigmented retina, as well as nests of undifferentiated embryonal carcinoma (EC) cells with high levels of alkaline phosphatase activity. The perigenital tumour in the hermaphrodite was a teratoma, less differentiated and with no evidence of EC cells. Glucose phosphate isomerase isozyme analysis indicated that both perigenital tumours were predominantly of the injected ES cell rather than the host blaslocyst type. The possible origins of these tumours, which are the first reported to have arisen from ES cells in chimaeric mice, are discussed.
DOI：
10.1073/pnas.78.12.7634
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARTIN, GR
通讯作者：
MARTIN, GR