Chinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth.
Chinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth.
复制标题
中草药的流产会影响缩减微环境和胎儿生长。
DOI：
10.1016/j.placenta.2015.02.006
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发表时间：
2015-05
期刊：
影响因子：
3.8
通讯作者：
Huang, S. J.
中科院分区：
文献类型：
--
作者：
Piao, L.;Chen, C. -P.;Yeh, C-C;Basar, M.;Masch, R.;Cheng, Y-C;Lockwood, C. J.;Schatz, F.;Huang, S. J.
胎儿宫内发育受限会使5%-10%的妊娠复杂化。本研究旨在验证中药配方JLFC01通过调节促炎症蜕膜微环境而影响妊娠和胎儿发育的假说。用抗CD68+抗CD86或抗CD163抗体对胎龄匹配的选择性流产或不全流产/稽留流产的蜕膜进行免疫组织化学染色。定量逆转录聚合酶链式反应和Luminex实验检测了JLFC01对IL-1β或肿瘤坏死因子α诱导的早孕蜕膜细胞因子表达的影响，以及对已建立的自然流产/宫内生长受限倾向的小鼠胎盘的影响。观察JLFC01对人子宫内膜内皮细胞血管生成的影响，包括平均管形面积、长度和分支点数目。记录用JLFC01处理的SA/IUGR倾向小鼠的摄食量、窝产仔数、胎儿体重、胎盘重量和吸收率。定量逆转录聚合酶链式反应、免疫印迹和免疫组织化学检测小鼠胎盘组织中IGF-I和IGF-IR的表达。在自然流产中，表达CD86和CD163的蜕膜巨噬细胞数量分别增加和减少。抑制早孕蜕膜细胞IL-1β、M-α、C-C基序配体2(CCL_2)、干扰素-γ诱导蛋白-10(IP-10)、CCL_5和IL-8的产生。JLFC01抑制IL-1、β或α处理的早孕蜕膜细胞增强巨噬细胞抑制的血管生成的活性。在SA/IUGR倾向的小鼠中，JLFC01增加了母亲的食物摄入量，增加了胎仔的大小，增加了胎儿和胎盘的重量，并降低了胎儿吸收率。JLFC01可诱导胎盘IGF-I和IGF-IR表达，抑制胎盘M-CSF、CCL2、CCL5、CCL11、CCL3和G-CSF的表达。JLFC01通过抑制蜕膜炎症，促进血管生成，促进胎儿生长，改善妊娠。
Intrauterine growth restriction complicates 5 - 10% of pregnancies. This study aims to test the hypothesis that Chinese herbal formula, JLFC01, affects pregnancy and fetal development by modulating the pro-inflammatory decidual micro-environment. Human decidua from gestational age-matched elective terminations or incomplete/missed abortion was immunostained using anti-CD68 + anti-CD86 or anti-CD163 antibodies. qRT-PCR and Luminex assay measured the effects of JLFC01 on IL-1β- or TNF-α-induced cytokine expression in first trimester decidual cells and on an established spontaneous abortion/intrauterine growth restriction (SA/IUGR)-prone mouse placentae. The effect of JLFC01 on human endometrial endothelial cell angiogenesis was evaluated by average area, length and numbers of branching points of tube formation. Food intake, litter size, fetal weight, placental weight and resorption rate were recorded in SA/IUGR-prone mouse treated with JLFC01. qRT-PCR, Western blot and immunohistochemistry assessed the expression of mouse placental IGF-I and IGF-IR. In spontaneous abortion, numbers of decidual macrophages expressing CD86 and CD163 are increased and decreased, respectively. JLFC01 reduces IL-1β- or TNF-α-induced GM-CSF, M-CSF, C-C motif ligand 2 (CCL2), interferon-γ-inducible protein-10 (IP-10), CCL5 and IL-8 production in first trimester decidual cells. JLFC01 suppresses the activity of IL-1β- or TNF-α-treated first trimester decidual cells in enhancing macrophage-inhibited angiogenesis. In SA/IUGR-prone mice, JLFC01 increases maternal food intake, litter size, fetal and placental weight, and reduces fetal resorption rate. JLFC01 induces IGF-I and IGF-IR expression and inhibits M-CSF, CCL2, CCL5, CCL11, CCL3 and G-CSF expression in the placentae. JLFC01 improves gestation by inhibiting decidual inflammation, enhancing angiogenesis and promoting fetal growth.
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影响因子：
7.5
作者：
Li M;Huang SJ
通讯作者：
Huang SJ
影响因子：
2.7
作者：
Longtine MS;Nelson DM
通讯作者：
Nelson DM
影响因子：
3.8
作者：
Holmes, R;Porter, H;Soothill, P
通讯作者：
Soothill, P
影响因子：
6
作者：
Huang, S. Joseph;Zenclussen, Ana C.;Lockwood, Charles J.
通讯作者：
Lockwood, Charles J.
DOI：
10.1084/jem.182.1.219
发表时间：
1995-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Luster AD;Greenberg SM;Leder P
通讯作者：
Leder P