Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses
Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses
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三种小核糖核酸病毒蛋白酶和基因组连锁蛋白基因组区域的分子克隆和序列测定
DOI：
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发表时间：
1986
影响因子：
5.4
通讯作者：
Sandoz Forschungsinstitut
中科院分区：
文献类型：
--
作者：
G. Werner;B. Rosenwirth;E. Bauer;J. Seifert;FRED;Werner;J. Besemer;Sandoz Forschungsinstitut
为了研究小核糖核酸病毒蛋白酶之间的相似性程度，我们克隆了肠道病毒埃可病毒9（株Barty）和两种鼻病毒（血清型1A和14LP）的基因组cdna，并确定了与脊髓灰质炎病毒相似的编码蛋白酶的区域的核苷酸序列。编码基因组连接蛋白VPg的区域的核苷酸序列，紧挨着蛋白酶，也被确定。核苷酸和推断的氨基酸序列与其他可用的小核糖核酸病毒序列的比较显示，蛋白酶和VPgs之间具有显著的同源性。在蛋白酶中鉴定出三个高度保守的肽区；其中一种对人小核糖核酸病毒是特异性的，在脑心肌炎病毒、口蹄疫病毒或豇豆花叶病毒蛋白酶中没有明显的对应物。在另外两个肽区，两个保守的氨基酸cys147和hys161可能是活性位点的活性残基。我们使用统计方法预测了二级结构的某些特征，如-螺旋、-薄片和转动，并发现许多这些构象是保守的。比较的蛋白酶的亲水特性也非常相似。因此，人小核糖核酸病毒的蛋白酶很可能具有类似的三维结构。
To investigate the degree of similarity between picornavirus proteases, we cloned the genomic cDNAs of an enterovirus, echovirus 9 (strain Barty), and two rhinoviruses, serotypes 1A and 14LP, and determined the nucleotide sequence of the region which, by analogy to poliovirus, encodes the protease. The nucleotide sequence of the region encoding the genome-linked protein VPg, immediately adjacent to the protease, was also determined. Comparison of nucleotide and deduced amino acid sequences with other available picornavirus sequences showed remarkable homology in proteases and among VPgs. Three highly conserved peptide regions were identified in the protease; one of these is specific for human picornaviruses and has no obvious counterpart in encephalomyocarditis virus, foot-and-mouth disease virus, or cowpea mosaic virus proteases. Within the other two peptide regions two conserved amino acids, Cys 147 and His 161, could be the reactive residues of the active site. We used a statistical method to predict certain features of the secondary structures, such as alpha helices, beta sheets, and turns, and found many of these conformations to be conserved. The hydropathy profiles of the compared proteases were also strikingly similar. Thus, the proteases of human picornaviruses very probably have a similar three-dimensional structure.
脊髓灰质炎病毒蛋白 P2-X 的多肽前体中的切割位点。
影响因子：
3.7
作者：
Semler,BL;Hanecak,R;Anderson,CW;Wimmer,E
通讯作者：
Wimmer,E
影响因子：
14.9
作者：
PALMENBERG, AC;KIRBY, EM;COLLETT, MS
通讯作者：
COLLETT, MS