Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells.
Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells.
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差异表达的miR-186-5p对大鼠前腺垂体细胞中FSH表达的调节
DOI：
10.1371/journal.pone.0194300
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Zhang JB
中科院分区：
文献类型：
--
作者：
Han DX;Xiao Y;Wang CJ;Jiang H;Gao Y;Yuan B;Zhang JB
卵泡刺激素（FSH）在动物生殖过程中起关键作用，包括精子发生和卵巢成熟。影响FSH分泌的因素很多。然而，尽管microRNA（miRNAs）通过调节靶基因发挥广泛的功能，但对其在FSH分泌中的作用知之甚少。我们先前的研究结果表明miR-186- 5 p靶向FSHb的3′ UTR;因此，我们研究了miR-186- 5 p是否可以调节大鼠前腺垂体细胞的FSH分泌。将miR-186- 5 p转染大鼠垂体前叶细胞。采用RT-qPCR和ELISA检测FSHb的表达和FSH的分泌。与对照组的表达相比，miR-186- 5 p模拟物降低了FSHb的表达，并减少了FSH的分泌。相比之下，FSH的mRNA水平和分泌均响应于miR-186- 5 p抑制剂而增加。我们的研究结果表明，miR-186- 5 p通过直接靶向FSHb 3′ UTR调节FSH分泌，为miRNAs在动物生殖调控中的重要性提供了额外的功能证据。
Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH secretion. Our previous results suggested that miR-186-5p targets the 3′ UTR of FSHb; therefore, we examined whether miR-186-5p could regulate FSH secretion in rat anterior adenohypophyseal cells. miR-186-5p was transfected into rat anterior pituitary cells. The expression of FSHb and the secretion of FSH were examined by RT-qPCR and ELISA. A miR-186-5p mimic decreased the expression of FSHb compared with expression in the control group and decreased FSH secretion. In contrast, both the mRNA levels and secretion of FSH increased in response to miR-186-5p inhibitors. Our results demonstrate that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3′ UTR, providing additional functional evidence for the importance of miRNAs in the regulation of animal reproduction.
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影响因子：
5.2
作者：
Huang, Tonghai;She, Kelin;He, Jianxing
通讯作者：
He, Jianxing
影响因子：
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作者：
Bottoni, A;Piccin, D;Uberti, ECD
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Uberti, ECD
影响因子：
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作者：
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通讯作者：
Zatelli MC
DOI：
10.1152/ajpendo.00616.2011
发表时间：
2012-04-01
影响因子：
5.1
作者：
Nemoto, Takahiro;Mano, Asuka;Shibasaki, Tamotsu
通讯作者：
Shibasaki, Tamotsu