Alignment of rat cardionatrin sequences with the preprocardionatrin sequence from complementary DNA.
Alignment of rat cardionatrin sequences with the preprocardionatrin sequence from complementary DNA.
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大鼠心钠素序列与互补 DNA 中的前心钠素序列的比对。
DOI：
10.1126/science.3157217
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发表时间：
1985
期刊：
影响因子：
56.9
通讯作者：
A. J. Bold
中科院分区：
文献类型：
--
作者：
T. Flynn;P. Davies;B. Kennedy;M. L. Bold;A. J. Bold
哺乳动物心房含有促进肾脏排出盐和水的肽。当在已知抑制蛋白水解的条件下提取大鼠心房组织时，会始终分离出四种利钠肽（心钠素 I 至 IV）。这些肽源自共同的前体，前心原蛋白，由 152 个氨基酸组成，其序列是通过互补 DNA 克隆的 DNA 测序确定的。氨基酸测序定位了整个序列中心钠素 I、III 和 IV 的起始点。心钠素 IV 与心钠素原最相似，因为它紧接在残基 25 处的信号序列之后开始。心钠素 III 从残基 73 处开始，之前测序的心钠素 I 从残基 123 处开始。组成分析表明，这些心钠素中的每一种都延伸至位置 150 处的酪氨酸，但缺少末端的两个精氨酸残基。
Mammalian atria contain peptides that promote the excretion of salt and water from the kidney. When rat atrial tissue is extracted under conditions known to inhibit proteolysis, four natriuretic peptides, cardionatrins I to IV, are consistently isolated. These peptides derive from a common precursor, preprocardionatrin, of 152 amino acids, whose sequence was determined by DNA sequencing of a complementary DNA clone. Amino acid sequencing located the start points of cardionatrins I, III, and IV in the overall sequence. Cardionatrin IV most closely resembles procardionatrin because it begins immediately after the signal sequence at residue 25. Cardionatrin III begins at residue 73, and cardionatrin I, sequenced previously, begins at residue 123. Compositional analysis indicated that each of these cardionatrins extends up to tyrosine at position 150 but lacks the terminal two arginine residues.
DOI：
10.1016/s0006-291x(84)80279-x
发表时间：
1984-03
影响因子：
3.1
作者：
K. Misono;H. Fukumi;R. Grammer;T. Inagami
通讯作者：
K. Misono;H. Fukumi;R. Grammer;T. Inagami
影响因子：
56.9
作者：
C. Seidman;A. Duby;E. Choi;R. Graham;E. Haber;C. Homcy;J. Smith;J. Seidman
通讯作者：
C. Seidman;A. Duby;E. Choi;R. Graham;E. Haber;C. Homcy;J. Smith;J. Seidman