Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
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DOI：
10.1083/jcb.149.4.901
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发表时间：
2000-05-15
期刊：
影响因子：
--
通讯作者：
Zerial M
中科院分区：
文献类型：
--
作者：
Sönnichsen B;De Renzis S;Nielsen E;Rietdorf J;Zerial M
已经描述了参与膜和质膜受体循环的两种内吞体群，早期的和循环的内吞体群。然而，这种区别主要基于货物分子的流动和这些膜在细胞内的空间分布。为了深入了解回收途径的膜组织，我们研究了运输机械的三个调节组件Rab4、Rab5和Rab11。继转铁蛋白作为货物分子和GFP标记的Rab蛋白之后，我们可以显示货物通过内体上不同的结构域移动。这些结构域被不同的Rab蛋白占据，揭示了同一连续膜内的区隔作用。内体由Rab4、Rab5和Rab11结构域的多种组合组成，这些结构域是动态的，但随着时间的推移不会明显混合。观察到三个主要种群：一个只包含Rab5，第二个包含Rab4和Rab5，第三个包含Rab4和Rab11。这些膜结构域表现出不同的药理敏感性，反映了它们的生化和功能多样性。我们认为，内小体是由不同的RAB结构域组成的马赛克，通过招募特定的效应蛋白来创建，这些蛋白协同作用在膜上产生一个受限的环境。
Two endosome populations involved in recycling of membranes and receptors to the plasma membrane have been described, the early and the recycling endosome. However, this distinction is mainly based on the flow of cargo molecules and the spatial distribution of these membranes within the cell. To get insights into the membrane organization of the recycling pathway, we have studied Rab4, Rab5, and Rab11, three regulatory components of the transport machinery. Following transferrin as cargo molecule and GFP-tagged Rab proteins we could show that cargo moves through distinct domains on endosomes. These domains are occupied by different Rab proteins, revealing compartmentalization within the same continuous membrane. Endosomes are comprised of multiple combinations of Rab4, Rab5, and Rab11 domains that are dynamic but do not significantly intermix over time. Three major populations were observed: one that contains only Rab5, a second with Rab4 and Rab5, and a third containing Rab4 and Rab11. These membrane domains display differential pharmacological sensitivity, reflecting their biochemical and functional diversity. We propose that endosomes are organized as a mosaic of different Rab domains created through the recruitment of specific effector proteins, which cooperatively act to generate a restricted environment on the membrane.
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DOI：
10.1083/jcb.109.6.2703
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Griffiths G;Back R;Marsh M
通讯作者：
Marsh M
影响因子：
64.5
作者：
Horiuchi, H;Lippe, R;Zerial, M
通讯作者：
Zerial, M
影响因子：
64.5
作者：
McBride, HM;Rybin, V;Zerial, M
通讯作者：
Zerial, M
DOI：
10.1083/jcb.109.6.3303
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dunn KW;McGraw TE;Maxfield FR
通讯作者：
Maxfield FR
影响因子：
56.9
作者：
Echard, A;Jollivet, F;Goud, B
通讯作者：
Goud, B