An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.
An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.
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DOI：
10.1016/j.cell.2013.02.037
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发表时间：
2013-03-28
期刊：
影响因子：
64.5
通讯作者：
Wolin SL
中科院分区：
文献类型：
--
作者：
Chen X;Taylor DW;Fowler CC;Galan JE;Wang HW;Wolin SL
许多细菌含有Ro自身抗原的直系同源物，Ro自身抗原是一种结合称为Y RNA的非编码RNA（ncRNA）的环状蛋白质。在唯一研究的细菌，耐辐射异常球菌中，Ro直系同源物Rsr的功能在热应激诱导的rRNA成熟和饥饿诱导的rRNA衰变。然而，这种保守的蛋白质及其相关的ncRNA的作用机制一直不清楚。我们报告说，Rsr和核糖核酸外切酶多核苷酸磷酸化酶（PNTR）形成一个RNA降解机器，是由Y RNA支架。单粒子电子显微镜，然后对接到重建的原子模型，表明Rsr通道单链RNA进入PNDR-cavity。生物化学分析表明，Rsr和Y RNA适应PNECs的有效降解结构RNA。Ro直系同源物和ncRNA也与鼠伤寒沙门氏菌中的PNTR相关。我们的研究确定了一种新的核糖核蛋白机器，并证明ncRNA，通过拴系蛋白质辅因子，可以改变酶的底物特异性。
Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In the only studied bacterium, Deinococcus radiodurans, the Ro ortholog Rsr functions in heat stress-induced rRNA maturation and starvation-induced rRNA decay. However, the mechanism by which this conserved protein and its associated ncRNAs act has been obscure. We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) form an RNA degradation machine that is scaffolded by Y RNA. Single-particle electron microscopy, followed by docking of atomic models into the reconstruction, suggests that Rsr channels single-stranded RNA into the PNPase cavity. Biochemical assays reveal that Rsr and Y RNA adapt PNPase for effective degradation of structured RNAs. A Ro ortholog and ncRNA also associate with PNPase in Salmonella Typhimurium. Our studies identify a new ribonucleoprotein machine and demonstrate that ncRNA, by tethering a protein cofactor, can alter the substrate specificity of an enzyme.
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