Maize WRKY Transcription Factor ZmWRKY106 Confers Drought and Heat Tolerance in Transgenic Plants.
Maize WRKY Transcription Factor ZmWRKY106 Confers Drought and Heat Tolerance in Transgenic Plants.
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DOI：
10.3390/ijms19103046
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发表时间：
2018-10-06
影响因子：
5.6
通讯作者：
Xu ZS
中科院分区：
文献类型：
--
作者：
Wang CT;Ru JN;Liu YW;Li M;Zhao D;Yang JF;Fu JD;Xu ZS
WRKY转录因子是植物中最大的转录因子家族之一，在植物生长发育、防御调节和逆境反应中发挥重要作用。然而，在玉米中对这个家族的了解是有限的。在本研究中，我们根据玉米干旱从头转录组的测序数据，鉴定了一个干旱诱导的WRKY基因ZmWRKY106。系统发育树分析表明，ZmWRKY106与OsWRKY13的亲缘关系较近。ZmWRKY106的亚细胞定位仅见于细胞核。ZmWRKY106的启动子区域包括C-重复/脱水反应元件(DRE)、低温反应元件(LTR)、MBS元件和TCA元件，这些元件可能参与干旱、寒冷和水杨酸(SA)胁迫反应。ZmWRKY106的表达受干旱、高温和外源脱落酸(ABA)的显著诱导，而受盐的诱导作用较弱。过表达ZmWRKY106通过ABA信号通路调节胁迫相关基因，提高了转基因拟南芥的耐旱性和耐热性，并通过提高干旱胁迫下超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化氢酶(POD)的活性，降低了转基因植株中的ROS含量。这表明ZmWRKY106参与了多种非生物胁迫反应途径，在干旱和高温胁迫下起到了积极的作用。
WRKY transcription factors constitute one of the largest transcription factor families in plants, and play crucial roles in plant growth and development, defense regulation and stress responses. However, knowledge about this family in maize is limited. In the present study, we identified a drought-induced WRKY gene, ZmWRKY106, based on the maize drought de novo transcriptome sequencing data. ZmWRKY106 was identified as part of the WRKYII group, and a phylogenetic tree analysis showed that ZmWRKY106 was closer to OsWRKY13. The subcellular localization of ZmWRKY106 was only observed in the nucleus. The promoter region of ZmWRKY106 included the C-repeat/dehydration responsive element (DRE), low-temperature responsive element (LTR), MBS, and TCA-elements, which possibly participate in drought, cold, and salicylic acid (SA) stress responses. The expression of ZmWRKY106 was induced significantly by drought, high temperature, and exogenous abscisic acid (ABA), but was weakly induced by salt. Overexpression of ZmWRKY106 improved the tolerance to drought and heat in transgenic Arabidopsis by regulating stress-related genes through the ABA-signaling pathway, and the reactive oxygen species (ROS) content in transgenic lines was reduced by enhancing the activities of superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) under drought stress. This suggested that ZmWRKY106 was involved in multiple abiotic stress response pathways and acted as a positive factor under drought and heat stress.
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DOI：
10.1186/s12284-016-0137-y
发表时间：
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